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Literature summary for 4.1.1.35 extracted from

  • Wheatley, E.R.; Davies, D.R.; Bolwell, G.P.
    Characterisation and immunolocation of an 87 kDa polypeptide associated with UDP-glucuronic acid decarboxylase activity from differentiating tobacco cells (Nicotiana tabacum L.) (2002), Phytochemistry, 61, 771-780.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
transformed with the ipt gene from Agrobacterium tumefaciens Nicotiana tabacum

General Stability

General Stability Organism
repeated freeze and thawing of samples results in significant loss of activity Nicotiana tabacum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.767
-
UDP-glucuronic acid pH 7.0, 37°C Nicotiana tabacum

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Nicotiana tabacum 5829
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
87000
-
? * 87000, SDS-PAGE Nicotiana tabacum
220000
-
gel filtration Nicotiana tabacum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-D-glucuronate Nicotiana tabacum biosynthesis of cell wall polysaccharides and glycosylation of proteins UDP-D-xylose + CO2
-
ir

Organism

Organism UniProt Comment Textmining
Nicotiana tabacum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Nicotiana tabacum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
17.57
-
-
Nicotiana tabacum

Storage Stability

Storage Stability Organism
-70°C, activity is not effected by storage for several months Nicotiana tabacum
4°C, stable Nicotiana tabacum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-D-glucuronate
-
Nicotiana tabacum UDP-D-xylose + CO2
-
?
UDP-D-glucuronate biosynthesis of cell wall polysaccharides and glycosylation of proteins Nicotiana tabacum UDP-D-xylose + CO2
-
ir

Subunits

Subunits Comment Organism
? ? * 87000, SDS-PAGE Nicotiana tabacum

Synonyms

Synonyms Comment Organism
UDP-glucuronate acid decarboxylase
-
Nicotiana tabacum
UDP-glucuronic acid decarboxylase
-
Nicotiana tabacum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Nicotiana tabacum

pH Range

pH Minimum pH Maximum Comment Organism
5.5 8
-
Nicotiana tabacum

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Nicotiana tabacum