Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3)CodonPlus RIL cells and Rosetta2(DE3)pLysS cells | Thermococcus kodakarensis |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion method, using 100 mM acetate buffer (pH 6.0), 80-100 mM CaCl2, 5-6% (w/v) polyethylene glycol 6,000, and 10% (v/v) 2-methylpentane-2,4-diol | Thermococcus kodakarensis |
Protein Variants | Comment | Organism |
---|---|---|
V330T | the mutant shows increased activity and reduced thermal stability compared to the wild type enzyme | Thermococcus kodakarensis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-carboxy-D-arabinitol 1,5-bisphosphate | - |
Thermococcus kodakarensis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.06 | - |
CO2 | wild type enzyme, in 100 mM Bicine-NaOH (pH 8.3) and 10 mM MgCl2, temperature not specified in the publication | Thermococcus kodakarensis | |
0.067 | - |
CO2 | mutant enzyme V330T, in 100 mM Bicine-NaOH (pH 8.3) and 10 mM MgCl2, temperature not specified in the publication | Thermococcus kodakarensis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | the active site contains a Mg2+ ion | Thermococcus kodakarensis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
x * 50000, estimated from amino acid sequence | Thermococcus kodakarensis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermococcus kodakarensis | O93627 | - |
- |
Purification (Comment) | Organism |
---|---|
anion exchange column chromatography and Superdex 200 gel filtration | Thermococcus kodakarensis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-ribulose 1,5-bisphosphate + CO2 + H2O | - |
Thermococcus kodakarensis | 3-phospho-D-glycerate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 50000, estimated from amino acid sequence | Thermococcus kodakarensis |
Synonyms | Comment | Organism |
---|---|---|
ribulose-1,5-bisphosphate carboxylase/oxygenase | - |
Thermococcus kodakarensis |
type III Rubisco | - |
Thermococcus kodakarensis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
90 | 100 | the wild type enzyme shows half-lives of 220 min at 90°C and 48 min at 100°C | Thermococcus kodakarensis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.31 | - |
CO2 | wild type enzyme, in 100 mM Bicine-NaOH (pH 8.3) and 10 mM MgCl2, temperature not specified in the publication | Thermococcus kodakarensis | |
0.53 | - |
CO2 | mutant enzyme V330T, in 100 mM Bicine-NaOH (pH 8.3) and 10 mM MgCl2, temperature not specified in the publication | Thermococcus kodakarensis |