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Literature summary for 4.1.1.4 extracted from
- Conversion of levulinic acid to 2-butanone by acetoacetate decarboxylase from Clostridium acetobutylicum (2013), Appl. Microbiol. Biotechnol., 97, 5627-5634.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 2,2-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) | activates about 2fold at 5-10 mM | Clostridium acetobutylicum |  |
| methyl viologen | activates 6.7fold at 10 mM | Clostridium acetobutylicum | |
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | acetoacetate decarboxylase from Clostridium acetobutylicum can act as a biocatalyst for decarboxylation of levulinic acid in an enzymatic system for synthesis of 2-butanone from levulinic acid | Clostridium acetobutylicum |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3), best induction over 24 h at 20°C with 1 mM IPTG | Clostridium acetobutylicum |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Azure B | slight inhibition at 2-10 mM | Clostridium acetobutylicum | |
| levulinic acid | substrate inhibition is observed with levulinic acid concentration higher than 5 mM | Clostridium acetobutylicum | |
| methylene blue | complete inhibition at 2 mM | Clostridium acetobutylicum | |
| Zn2+ | inhibits at above 5 mM | Clostridium acetobutylicum | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetoacetate + H+ | Clostridium acetobutylicum | - |
acetone + CO2 | - |
? | |
| additional information | Clostridium acetobutylicum | acetoacetate decarboxylase from Clostridium acetobutylicum can act as a biocatalyst for decarboxylation of levulinic acid | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Clostridium acetobutylicum | P23670 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Clostridium acetobutylicum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetoacetate + H+ | - |
Clostridium acetobutylicum | acetone + CO2 | - |
? | |
| levulinic acid + H+ | - |
Clostridium acetobutylicum | 2-butanone + CO2 | - |
? | |
| additional information | acetoacetate decarboxylase from Clostridium acetobutylicum can act as a biocatalyst for decarboxylation of levulinic acid | Clostridium acetobutylicum | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AADC | - |
Clostridium acetobutylicum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
- |
Clostridium acetobutylicum |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 45 | over 30% of maximal activity at 20°C and 45°C | Clostridium acetobutylicum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
with levulinic acid | Clostridium acetobutylicum |
| 6 | - |
with acetoacetate | Clostridium acetobutylicum |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 3.5 | 8 | activity range, profile overview | Clostridium acetobutylicum |
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