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Literature summary for 4.1.1.49 extracted from
- The phosphoenolpyruvate carboxykinase of Trypanosoma (Schizotrypanum) cruzi epimastigotes: molecular, kinetic, and regulatory properties (1987), Arch. Biochem. Biophys., 258, 186-195.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| DTNB | - |
Trypanosoma cruzi |  |
| p-chloromercuribenzoate | - |
Trypanosoma cruzi | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.027 | - |
oxaloacetate | - |
Trypanosoma cruzi | |
| 0.039 | - |
ADP | ADP in form of MgADP- | Trypanosoma cruzi | |
| 0.36 | - |
phosphoenolpyruvate | - |
Trypanosoma cruzi | |
| 3.7 | - |
CO2 | CO2 in form of HCO3- | Trypanosoma cruzi | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mn2+ | one Mn2+ is required for the activation of each 42000 Da subunit | Trypanosoma cruzi | |
| Mn2+ | strictly dependent on | Trypanosoma cruzi | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 42000 | - |
2 * 42000, SDS-PAGE | Trypanosoma cruzi |
| 82000 | - |
gel filtration | Trypanosoma cruzi |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Trypanosoma cruzi | important regulatory function of the enzyme in the amino-acid catabolism of Trypanosoma cruzi | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Trypanosoma cruzi | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Trypanosoma cruzi |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| epimastigote | - |
Trypanosoma cruzi | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Trypanosoma cruzi |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ADP + phosphoenolpyruvate + CO2 | - |
Trypanosoma cruzi | ATP + oxaloacetate | - |
? | |
| additional information | important regulatory function of the enzyme in the amino-acid catabolism of Trypanosoma cruzi | Trypanosoma cruzi | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 42000, SDS-PAGE | Trypanosoma cruzi |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.9 | - |
carboxylation | Trypanosoma cruzi |
| 8 | - |
decarboxylation | Trypanosoma cruzi |
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