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Literature summary for 4.1.1.49 extracted from

  • Yevenes, A.; Espinoza, R.; Rivas-Pardo, J.A.; Villarreal, J.M.; Gonzalez-Nilo, F.D.; Cardemil, E.
    Site-directed mutagenesis study of the microenvironment characteristics of Lys213 of Saccharomyces cerevisiae phosphoenolpyruvate carboxykinase (2006), Biochimie, 88, 663-672.
    View publication on PubMed
Search for more literature for 4.1.1.49

Cloned(Commentary)

Cloned (Comment) Organism
cloned in Escherichia coli and in in the PEP carboxykinase-deficient Saccharomyces cerevisiae-strain PUK-3B Saccharomyces cerevisiae

Protein Variants

Protein Variants Comment Organism
F216Y kinetic parameter in the PEP carboxylation direction: Km: 0.170 mM (MnADP-), 0.917 mM (phosphoenolpyruvate), 0.056 mM (Mn2+), 0.024 mM (HCO3-) Saccharomyces cerevisiae
F216Y kinetic parameter in the PEP formation direction: Km: 0.016 mM (MnATP2-), 1.079 mM (oxalacetate), 0.345 mM (Mn2+) Saccharomyces cerevisiae
F216Y mutation Phe216Tyr shows that the introduction of a hydroxyl group in the lateral chain of the residue produces a substantial loss in the enzyme affinity for Mn2+, suggesting an increase of the pKa of Lys213 Saccharomyces cerevisiae
F216Y pH: 6.5: Km: 0.073 mM (Mn2+), pH: 8.1: Km: 0.035 mM Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.01
-
 Mn2+ kinetic parameter in the PEP formation direction Saccharomyces cerevisiae
0.012
-
 Mn2+ pH high (pH range:6-8.4), pH:12, PEP carboxylation direction Saccharomyces cerevisiae
0.016
-
 ATP F216Y: kinetic parameter in the PEP formation direction Saccharomyces cerevisiae
0.016
-
 ATP ATP in form of MnATP2- Saccharomyces cerevisiae
0.018
-
 CO2 kinetic parameter in the PEP carboxylation direction Saccharomyces cerevisiae
0.018
-
 CO2 CO2 in form of HCO3- Saccharomyces cerevisiae
0.023
-
 Mn2+ kinetic parameter in the PEP carboxylation direction Saccharomyces cerevisiae
0.024
-
 CO2 F216Y: kinetic parameter in the PEP carboxylation direction Saccharomyces cerevisiae
0.024
-
 CO2 CO2 in form of HCO3- Saccharomyces cerevisiae
0.034
-
 ADP kinetic parameter in the PEP carboxylation direction Saccharomyces cerevisiae
0.034
-
 ATP kinetic parameter in the PEP formation direction Saccharomyces cerevisiae
0.034
-
 ATP ATP in form of MnATP2- Saccharomyces cerevisiae
0.034
-
 ADP ADP in form of MnADP- Saccharomyces cerevisiae
0.056
-
 Mn2+ F216Y: kinetic parameter in the PEP carboxylation direction Saccharomyces cerevisiae
0.066
-
 oxalacetate kinetic parameter in the PEP formation direction Saccharomyces cerevisiae
0.123
-
 Mn2+ pH low (pH range:6-8.4), PEP carboxylation direction Saccharomyces cerevisiae
0.17
-
 ADP F216Y: kinetic parameter in the PEP carboxylation direction Saccharomyces cerevisiae
0.17
-
 ADP ADP in form of MnADP- Saccharomyces cerevisiae
0.307
-
 phosphoenolpyruvate kinetic parameter in the PEP carboxylation direction Saccharomyces cerevisiae
0.345
-
 Mn2+ F216Y: kinetic parameter in the PEP formation direction Saccharomyces cerevisiae
0.917
-
 phosphoenolpyruvate F216Y: kinetic parameter in the PEP carboxylation direction Saccharomyces cerevisiae
1.079
-
 oxalacetate F216Y: kinetic parameter in the PEP formation direction Saccharomyces cerevisiae

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ it is shown that Mn2+ affinity increases as the pH increases Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP + phosphoenolpyruvate + CO2
-
 Saccharomyces cerevisiae ATP + oxaloacetate
-
 r
ATP + oxaloacetate
-
 Saccharomyces cerevisiae ADP + phosphoenolpyruvate + CO2
-
 r

Synonyms

Synonyms Comment Organism
PEP carboxykinase
-
 Saccharomyces cerevisiae
phosphoenolpyruvate carboxykinase
-
 Saccharomyces cerevisiae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.6
-
 assay in the pyruvate kinase-like reaction Saccharomyces cerevisiae
6.9
-
 assay in the carboxylation direction Saccharomyces cerevisiae
7
-
 assay in the PEP formation direction Saccharomyces cerevisiae

pH Range

pH Minimum pH Maximum Comment Organism
additional information
-
 it is shown that Mn2+ affinity increases as the pH increases Saccharomyces cerevisiae