Cloned (Comment) | Organism |
---|---|
transformed into the PEP carboxykinase-deficient yeast strain PUK-3B (MATalpha pck ura3) | Saccharomyces cerevisiae |
Protein Variants | Comment | Organism |
---|---|---|
R457M | site directed mutagenesis | Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.009 | - |
CO2 | for wild-type | Saccharomyces cerevisiae | |
0.009 | - |
CO2 | CO2 in form of HCO3- | Saccharomyces cerevisiae | |
0.0098 | - |
CO2 | for R457M mutant | Saccharomyces cerevisiae | |
0.0098 | - |
CO2 | CO2 in form of HCO3- | Saccharomyces cerevisiae | |
0.04 | - |
ADP | for wild-type | Saccharomyces cerevisiae | |
0.04 | - |
ADP | ADP in form of MnADP- | Saccharomyces cerevisiae | |
0.3 | - |
phosphoenolpyruvate | for R457M mutant | Saccharomyces cerevisiae | |
0.31 | - |
phosphoenolpyruvate | for wild-type | Saccharomyces cerevisiae | |
2.03 | - |
ADP | for R457M mutant | Saccharomyces cerevisiae | |
2.03 | - |
ADP | ADP in form of MnADP- | Saccharomyces cerevisiae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Saccharomyces cerevisiae | |
Mn2+ | - |
Saccharomyces cerevisiae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
251000 | - |
calculated | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | P10963 | - |
- |
Purification (Comment) | Organism |
---|---|
SDS-PAGE | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP + phosphoenolpyruvate + CO2 | - |
Saccharomyces cerevisiae | ATP + oxaloacetate | - |
r |
Subunits | Comment | Organism |
---|---|---|
tetramer | - |
Saccharomyces cerevisiae |
Synonyms | Comment | Organism |
---|---|---|
ATP: oxaloacetate carboxy-lyase | - |
Saccharomyces cerevisiae |
PEP carboxykinase | - |
Saccharomyces cerevisiae |
phosphoenolpyruvate carboxykinase | - |
Saccharomyces cerevisiae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3 | 6 | phosphoenolpyruvate | for wild-type | Saccharomyces cerevisiae | |
13 | - |
phosphoenolpyruvate | for R457M mutant | Saccharomyces cerevisiae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ADP | preference for ADP (or ATP) over other nucleotides | Saccharomyces cerevisiae | |
ATP | preference for ADP (or ATP) over other nucleotides | Saccharomyces cerevisiae |