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Literature summary for 4.1.1.5 extracted from

  • Phalip, V.; Monnet, C.; Schmitt, P.; Renault, P.; Godon, J.J.; Divies, C.
    Purification and properties of the alpha-acetolactate decarboxylase from Lactococcus lactis subsp. lactis NCDO 2118 (1994), FEBS Lett., 351, 95-99.
    View publication on PubMed
Search for more literature for 4.1.1.5

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in Escherichia coli Lactococcus lactis

General Stability

General Stability Organism
very unstable, especially when diluted, at 0.05 mg/ml the activity is totally lost within 24 h at 4°C. Addition of bovine serum albumin, 5 mg/ml, allows the preservation of the activity for at least 2 weeks Lactococcus lactis

Inhibitors

Inhibitors Comment Organism Structure
EDTA 2 mM Mn2+ restores 82% of the activity, 2 mM Zn2+ restores 69% of the initial activity Lactococcus lactis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
10
-
 2-Hydroxy-2-methyl-3-oxobutanoate in presence of Leu Lactococcus lactis
75
-
 2-Hydroxy-2-methyl-3-oxobutanoate in absence of Leu Lactococcus lactis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
26500
-
 6 * 26500, SDS-PAGE Lactococcus lactis
150000
-
 gel filtration Lactococcus lactis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-Hydroxy-2-methyl-3-oxobutanoate Lactococcus lactis activation by Leu, Val and Ile ?
-
 ?
2-Hydroxy-2-methyl-3-oxobutanoate Lactococcus lactis NCDO 2118 activation by Leu, Val and Ile ?
-
 ?

Organism

Organism UniProt Comment Textmining
Lactococcus lactis
-
-
-
Lactococcus lactis
-
 subsp. lactis
-
Lactococcus lactis NCDO 2118
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Lactococcus lactis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
454.3
-
-
 Lactococcus lactis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-Hydroxy-2-methyl-3-oxobutanoate
-
 Lactococcus lactis (R)-3-Hydroxybutan-2-one + CO2
-
 ?
2-Hydroxy-2-methyl-3-oxobutanoate
-
 Lactococcus lactis NCDO 2118 (R)-3-Hydroxybutan-2-one + CO2
-
 ?
2-Hydroxy-2-methyl-3-oxobutanoate activation by Leu, Val and Ile Lactococcus lactis ?
-
 ?
2-Hydroxy-2-methyl-3-oxobutanoate activation by Leu, Val and Ile Lactococcus lactis NCDO 2118 ?
-
 ?

Subunits

Subunits Comment Organism
hexamer 6 * 26500, SDS-PAGE Lactococcus lactis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Lactococcus lactis

pH Range

pH Minimum pH Maximum Comment Organism
5.4 6.9 75% of maximal activity at pH 5.4 and at pH 6.9 Lactococcus lactis