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Literature summary for 4.1.1.5 extracted from
- Improving the acidic stability of Staphylococcus aureus alpha-acetolactate decarboxylase in Bacillus subtilis by changing basic residues to acidic residues (2015), Amino Acids, 47, 707-717 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Bacillus subtilis strain WB600 | Staphylococcus aureus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| K52D | the mutant has almost no variation on enzyme activity, but exhibits improved stability over pH 4.0-5.0 compared to the wild type enzyme | Staphylococcus aureus |
| K52E | the mutant has almost no variation on enzyme activity, but exhibits improved stability over pH 4.0-5.0 compared to the wild type enzyme | Staphylococcus aureus |
| N43D | the mutant has almost no variation on enzyme activity, but exhibits improved stability over pH 4.0-5.0 compared to the wild type enzyme | Staphylococcus aureus |
| N43D/K52D | the mutant shows dramatically improved acidic stability with prolonged half-life of 3.5 h (compared to the wild type of 1.5 h) at pH 4.0 | Staphylococcus aureus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0177 | - |
(2S)-2-hydroxy-2-methyl-3-oxobutanoate | at pH 6.0 and 45°C | Staphylococcus aureus |  |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (2S)-2-hydroxy-2-methyl-3-oxobutanoate | Staphylococcus aureus | - |
(3R)-3-hydroxybutan-2-one + CO2 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Staphylococcus aureus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA column chromatography | Staphylococcus aureus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (2S)-2-hydroxy-2-methyl-3-oxobutanoate | - |
Staphylococcus aureus | (3R)-3-hydroxybutan-2-one + CO2 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 28000, SDS-PAGE | Staphylococcus aureus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ALDC | - |
Staphylococcus aureus |
| alpha-Acetolactate decarboxylase | - |
Staphylococcus aureus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
- |
Staphylococcus aureus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 50 | more than 60% activity between 30 and 50°C | Staphylococcus aureus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
the enzyme activity is nearly 90% at -20°C and about 80% from 0 to 35°C after 10 h, while rapid inactivation is observed over 45°C | Staphylococcus aureus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
- |
Staphylococcus aureus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 9 | the wild type enzyme has a half-life of 1.5 h at pH 4.0. The enzyme retains more than 75 % activity at pH 7.0-9.0 after preincubation for 10 h, while the enzyme activity at pH 6.0 is 41% after 10 h and decreases much more rapidly at pH 5.0 after 2.5 h | Staphylococcus aureus |
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Release 2023.1 (January 2023)
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