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Literature summary for 4.1.1.61 extracted from

  • Lack, A.; Fuchs, G.
    Carboxylation of phenylphosphate by phenol carboxylase, an enzyme system of anaerobic phenol metabolism (1992), J. Bacteriol., 174, 3629-3636.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.2
-
Phenylphosphate CO2:4-hydroxybenzoate isotope exchange reaction Pseudomonas sp.
0.67
-
4-hydroxybenzoate
-
Pseudomonas sp.
1.5
-
CO2 dissolved Pseudomonas sp.

Metals/Ions

Metals/Ions Comment Organism Structure
K+ not required for phenylphosphate decarboxylase activity Pseudomonas sp.
Mn2+ required for phenylphosphate decarboxylase activity Pseudomonas sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
280000
-
gel filtration Pseudomonas sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Pseudomonas sp. induced after anaerobic growth on phenol ?
-
?
additional information Pseudomonas sp. K172 induced after anaerobic growth on phenol ?
-
?
Phenol + CO2 Pseudomonas sp. first step in the anaerobic metabolism of phenol ?
-
?
Phenol + CO2 Pseudomonas sp. K172 first step in the anaerobic metabolism of phenol ?
-
?

Organism

Organism UniProt Comment Textmining
Pseudomonas sp.
-
-
-
Pseudomonas sp. K172
-
-
-

Oxidation Stability

Oxidation Stability Organism
half-life in air-saturated solution: less than 1 min Pseudomonas sp.

Reaction

Reaction Comment Organism Reaction ID
4-hydroxybenzoate = phenol + CO2 biological Kolbe-Schmitt carboxylation. The formation of an enzyme-bound phenolate anion from the activated phenolic compound is the rate-determining step in the carboxylation reaction Pseudomonas sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.012
-
phenylphosphate carboxylation Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-Hydroxybenzoate
-
Pseudomonas sp. Phenol + CO2
-
?
4-Hydroxybenzoate
-
Pseudomonas sp. K172 Phenol + CO2
-
?
additional information induced after anaerobic growth on phenol Pseudomonas sp. ?
-
?
additional information induced after anaerobic growth on phenol Pseudomonas sp. K172 ?
-
?
Phenol + CO2
-
Pseudomonas sp. 4-Hydroxybenzoate
-
?
Phenol + CO2
-
Pseudomonas sp. K172 4-Hydroxybenzoate
-
?
Phenol + CO2 first step in the anaerobic metabolism of phenol Pseudomonas sp. ?
-
?
Phenol + CO2 first step in the anaerobic metabolism of phenol Pseudomonas sp. K172 ?
-
?
Phenylphosphate + CO2
-
Pseudomonas sp. 4-Hydroxybenzoate + phosphate
-
?
Phenylphosphate + CO2
-
Pseudomonas sp. K172 4-Hydroxybenzoate + phosphate
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
phenylphosphate carboxylation Pseudomonas sp.