KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.2 | - |
Phenylphosphate | CO2:4-hydroxybenzoate isotope exchange reaction | Pseudomonas sp. | |
0.67 | - |
4-hydroxybenzoate | - |
Pseudomonas sp. | |
1.5 | - |
CO2 | dissolved | Pseudomonas sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | not required for phenylphosphate decarboxylase activity | Pseudomonas sp. | |
Mn2+ | required for phenylphosphate decarboxylase activity | Pseudomonas sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
280000 | - |
gel filtration | Pseudomonas sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Pseudomonas sp. | induced after anaerobic growth on phenol | ? | - |
? | |
additional information | Pseudomonas sp. K172 | induced after anaerobic growth on phenol | ? | - |
? | |
Phenol + CO2 | Pseudomonas sp. | first step in the anaerobic metabolism of phenol | ? | - |
? | |
Phenol + CO2 | Pseudomonas sp. K172 | first step in the anaerobic metabolism of phenol | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas sp. | - |
- |
- |
Pseudomonas sp. K172 | - |
- |
- |
Oxidation Stability | Organism |
---|---|
half-life in air-saturated solution: less than 1 min | Pseudomonas sp. |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
4-hydroxybenzoate = phenol + CO2 | biological Kolbe-Schmitt carboxylation. The formation of an enzyme-bound phenolate anion from the activated phenolic compound is the rate-determining step in the carboxylation reaction | Pseudomonas sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.012 | - |
phenylphosphate carboxylation | Pseudomonas sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-Hydroxybenzoate | - |
Pseudomonas sp. | Phenol + CO2 | - |
? | |
4-Hydroxybenzoate | - |
Pseudomonas sp. K172 | Phenol + CO2 | - |
? | |
additional information | induced after anaerobic growth on phenol | Pseudomonas sp. | ? | - |
? | |
additional information | induced after anaerobic growth on phenol | Pseudomonas sp. K172 | ? | - |
? | |
Phenol + CO2 | - |
Pseudomonas sp. | 4-Hydroxybenzoate | - |
? | |
Phenol + CO2 | - |
Pseudomonas sp. K172 | 4-Hydroxybenzoate | - |
? | |
Phenol + CO2 | first step in the anaerobic metabolism of phenol | Pseudomonas sp. | ? | - |
? | |
Phenol + CO2 | first step in the anaerobic metabolism of phenol | Pseudomonas sp. K172 | ? | - |
? | |
Phenylphosphate + CO2 | - |
Pseudomonas sp. | 4-Hydroxybenzoate + phosphate | - |
? | |
Phenylphosphate + CO2 | - |
Pseudomonas sp. K172 | 4-Hydroxybenzoate + phosphate | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
phenylphosphate carboxylation | Pseudomonas sp. |