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Literature summary for 4.1.1.7 extracted from
- Factors mediating activity, selectivity, and substrate specificity for the thiamin diphosphate-dependent enzymes benzaldehyde lyase and benzoylformate decarboxylase (2006), Chembiochem, 7, 1928-1934.
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| A460I | strongly decreased activity | Pseudomonas putida |
| A460I/F464I | strongly decreased activity | Pseudomonas putida |
| F464I | strongly decreased activity | Pseudomonas putida |
| H281A | strongly decreased activity | Pseudomonas putida |
| H70A | strongly decreased activity | Pseudomonas putida |
| S26A | strongly decreased activity | Pseudomonas putida |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.2 | - |
benzoylformate | mutant enzyme A460I | Pseudomonas putida |  |
| 0.4 | - |
benzoylformate | mutant enzyme H281A | Pseudomonas putida | |
| 0.8 | - |
benzoylformate | wild type enzyme | Pseudomonas putida | |
| 1.1 | - |
benzoylformate | mutant enzyme A460I/F464I | Pseudomonas putida | |
| 1.9 | - |
benzoylformate | mutant enzyme H70A | Pseudomonas putida | |
| 2.3 | - |
benzoylformate | mutant enzyme F464I | Pseudomonas putida | |
| 4.5 | - |
benzoylformate | mutant enzyme S26A | Pseudomonas putida | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas putida | P20906 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| benzaldehyde + benzaldehyde | - |
Pseudomonas putida | (R)-benzoin | - |
? | |
| benzoylformate | - |
Pseudomonas putida | benzaldehyde + CO2 | - |
? | |
| Benzoylformate + acetaldehyde | - |
Pseudomonas putida | (S)-2-Hydroxypropiophenone | - |
? | |
| additional information | does not perform the cleavage of 2-hydroxy ketones | Pseudomonas putida | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | - |
Pseudomonas putida |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| BFD | catalyzes a carboligase reaction that yields 2-hydroxypropiophenone, by using benzoylformate and acetaldehyde as substrates, and is able to catalyze the carboligation of a broad range of aldehydes | Pseudomonas putida |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| thiamine diphosphate | dependent | Pseudomonas putida | |
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