Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3)pLysS cells | Pseudomonas putida |
Crystallization (Comment) | Organism |
---|---|
in complex with (E)-2-oxo-4(pyridin-3-yl)-3-butenoic acid or 3-(pyridin-3-yl)acrylaldehyde, hanging drop vapour diffusion method, in 100 mM TrisHCl (pH 8.5), 150 mM CaCl2, 0.5% (v/v) MPD [2-methyl-2,4-pentanediol], and 22% (v/v) PEG 400 | Pseudomonas putida |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | P20906 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography | Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(E)-2-oxo-4(pyridin-3-yl)-3-butenoic acid | - |
Pseudomonas putida | 3-(pyridin-3-yl)acrylaldehyde + CO2 | - |
? | |
benzoylformate | - |
Pseudomonas putida | benzaldehyde + CO2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
BFDC | - |
Pseudomonas putida |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
thiamine diphosphate | dependent on | Pseudomonas putida |