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Literature summary for 4.1.1.71 extracted from

  • Shigeoka, S.; Onishi, T.; Maeda, K.; Nakano, Y.; Kitaoka, S.
    Occurence of thiamin pyrophopshate-dependent 2-oxoglutarate decarboxylase in mitochondria of Euglena gracilis (1986), FEBS Lett., 195, 43-47.
No PubMed abstract available
Search for more literature for 4.1.1.71

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol stimulates Euglena gracilis
DTT stimulates Euglena gracilis

Inhibitors

Inhibitors Comment Organism Structure
2-oxoglutarate above 2 mM substrate inhibition Euglena gracilis
Cu2+
-
 Euglena gracilis
Hg2+
-
 Euglena gracilis
NEM
-
 Euglena gracilis
Ni2+
-
 Euglena gracilis
PCMB
-
 Euglena gracilis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.33
-
 2-oxoglutarate pH 7.0, 30°C Euglena gracilis

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion exclusively Euglena gracilis 5739
-
additional information not: cytosol, microsomes Euglena gracilis
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ can partially replace Mg2+ in activation Euglena gracilis
Co2+ can partially replace Mg2+ in activation Euglena gracilis
MgCl2 required Euglena gracilis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Euglena gracilis the enzyme has a physiologically important role coupling with succinate-semialdehyde dehydrogenase in the tricarboxylic acid cycle ?
-
 ?

Organism

Organism UniProt Comment Textmining
Euglena gracilis
-
 lacking a 2-oxoglutarate dehydrogenase complex (EC 1.2.4.2 + EC 2.3.1.61 + EC 1.8.1.4)
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-oxoglutarate highly specific Euglena gracilis succinate semialdehyde + CO2
-
 ?
additional information the enzyme has a physiologically important role coupling with succinate-semialdehyde dehydrogenase in the tricarboxylic acid cycle Euglena gracilis ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Euglena gracilis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
35 40 35-40°C: activity maximum, maximum activity is maintained up to 60°C Euglena gracilis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
 complete loss of activity at 70°C Euglena gracilis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Euglena gracilis

Cofactor

Cofactor Comment Organism Structure
NADP+ stimulates Euglena gracilis
thiamine diphosphate required Euglena gracilis
thiamine diphosphate Km: 0.056 mM Euglena gracilis