Literature summary for 4.1.1.90 extracted from

Rishavy, M.A.; Hallgren, K.W.; Yakubenko, A.V.; Shtofman, R.L.; Runge, K.W.; Berkner, K.L.
Bronsted analysis reveals Lys218 as the carboxylase active site base that deprotonates vitamin K hydroquinone to initiate vitamin K-dependent protein carboxylation (2006), Biochemistry, 45, 13239-13248.

Search for more literature for 4.1.1.90

Cloned(Commentary)

Cloned (Comment)	Organism
mutational analysis is performed using an expression system lacking endogenous carboxylase. Construction of baculo viruses containing FLAG-tagged carboxylase mutants and expression in infected SF21 cells. Expresses the 758 amino acid human VKD carboxylase bearing a C-terminal extension of AAADYKDDDDK, where the last eight amino acids are the FLAG epitope	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
H160A	His to Ala mutants all show full epoxidase activity	Homo sapiens
H287A	His to Ala mutants all show full epoxidase activity	Homo sapiens
H381A	His to Ala mutants all show full epoxidase activity	Homo sapiens
K218A	K218A activity is not detectable. The addition of exogenous amines restores K218A activity while having little effect on wild type carboxylase	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
affinity chromatography	Homo sapiens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.0000026	-	H160A mutant	Homo sapiens
0.0000027	-	wild type	Homo sapiens
0.0000045	-	H381A mutant	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
L-glutamate + CO2 + O2 + vitamin K hydroquinone	-	Homo sapiens	gamma-carboxy L-glutamate + vitamin K epoxide + H2O	-	?

Synonyms

Synonyms	Comment	Organism
vitamin K-dependent carboxylase	-	Homo sapiens
VKD carboxylase	-	Homo sapiens

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	-	Homo sapiens
8.5	-	assay at	Homo sapiens

pH Range

pH Minimum	pH Maximum	Comment	Organism
5.5	8.5	-	Homo sapiens

Cofactor

Cofactor	Comment	Organism	Structure
vitamin K	-	Homo sapiens

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Release 2023.1 (January 2023)