Inhibitors | Comment | Organism | Structure |
---|---|---|---|
FFRCK | peptide, protease inhibitor | Bos taurus | |
FPRCK | peptide, protease inhibitor | Bos taurus | |
protease inhibitor mixture | PIC, freshly prepared as a 10x PIC stock containing 20 mM dithiothreitol, 20 mM EDTA, FFRCK (1.25 microg/ml), FPRCK (1.25 microg/ml), leupeptin (5 microg/ml), pepstatin A (7 microg/ml), phenylmethylsulfonyl fluoride (340 microg/ml), aprotinin (20 microg/ml) | Bos taurus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
microsome | - |
Bos taurus | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
94000 | - |
SDS-PAGE | Bos taurus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | Q07175 | - |
- |
Purification (Comment) | Organism |
---|---|
degree of purification is about 7000fold with reference to ammonium sulfate-fractionated microsomal protein from liver. Purification of carboxylase, solubilized microsomes: 281000 cpm/mg/h, flow-through of Affi-FIXQ/S: 200000 cpm/mg/h, bound to Affi-FIXQ/S: 140000000 cpm/mg/h, affinity-purified carboxylase: 1930000000 cpm/mg/h | Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Bos taurus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
FLEEL + CO2 + O2 + vitamin KH2 | Phe-Leu-Glu-Glu-Leu | Bos taurus | ? + vitamin K epoxide + H2O | - |
? | |
L-glutamate + CO2 + O2 + vitamin K hydroquinone | catalyzes modification of specific glutamic acids to gamma-carboxyglutamic acid in several blood-coagulation proteins | Bos taurus | gamma-carboxy-L-glutamate + vitamin K epoxide + H2O | - |
? | |
proFIXl9 + CO2 + O2 + vitamin KH2 | AVFLDHENANKILNRPKRY | Bos taurus | ? + vitamin K epoxide + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 94000, SDS-PAGE | Bos taurus |
Synonyms | Comment | Organism |
---|---|---|
glutamate carboxylase | - |
Bos taurus |
vitamin K-dependent carboxylase | - |
Bos taurus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
vitamin K | - |
Bos taurus |