Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ammonium sulfate | - |
Homo sapiens | |
proFIX 19 | - |
Homo sapiens |
Cloned (Comment) | Organism |
---|---|
expression of the cloned cDNA results in an increase in carboxylase activity in microsomes of transfected cells compared to mock-transfected cells | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | 758 residue integral membrane protein | Homo sapiens | 16020 | - |
microsome | microsomal carboxylase activity is compared from cells transfected with pCMV5 | Homo sapiens | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
87540 | - |
calculated from sequence | Homo sapiens |
94000 | - |
SDS-PAGE | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Homo sapiens | enzyme accomplishes the post-translational modification required for the activity of all of the vitamin K-dependent proteins | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P38435 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
HEK-293 cell | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
FLEEL + CO2 + O2 + ammonium sulfate | carboxylase activity is measured by 14CO2 incorporation into the synthetic peptide substrate FLEEL | Homo sapiens | ? + vitamin K epoxide + H2O | - |
? | |
FLEEL + CO2 + O2 + proFIX 19 | carboxylase activity is measured by 14CO2 incorporation into the synthetic peptide substrate FLEEL | Homo sapiens | ? + vitamin K epoxide + H2O | - |
? | |
FLEEL + CO2 + O2 + vitamin KH2 | carboxylase activity is measured by 14CO2 incorporation into the synthetic peptide substrate FLEEL | Homo sapiens | ? + vitamin K epoxide + H2O | - |
? | |
L-glutamate + CO2 + O2 + vitamin K hydroquinone | reaction is essential for the activity of all of the vitamin K-dependent proteins | Homo sapiens | gamma-carboxy-L-glutamate + vitamin K epoxide + H2O | - |
? | |
additional information | enzyme accomplishes the post-translational modification required for the activity of all of the vitamin K-dependent proteins | Homo sapiens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 94000, SDS-PAGE | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
gamma-glutamyl carboxylase | - |
Homo sapiens |
glutamate carboxylase | - |
Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
vitamin K | carboxylase and soybean seed lipoxygenase share 19.3% identity over a span of 198 amino acids, from residues 468 to 666 of carboxylase. This is interesting because the carboxylase acts as an oxygenase on the cofactor vitamin K-hydroquinone, and the similarity occurs in that region of the carboxylase likely to have enzymatic function | Homo sapiens |