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Literature summary for 4.1.2.13 extracted from

  • Zgiby, S.M.; Thomson, G.J.; Qamar, S.; Berry, A.
    Exploring substrate binding and discrimination in fructose1, 6-bisphosphate and tagatose 1,6-bisphosphate aldolases (2000), Eur. J. Biochem., 267, 1858-1868.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
-
Escherichia coli

Protein Variants

Protein Variants Comment Organism
K325A decreased kcat Escherichia coli
N35A 1.56% activity of the wild-type enzyme Escherichia coli
Q59A no significant effect on enzyme function Escherichia coli
S61A increased Km for fructose 1,6-bisphosphate Escherichia coli
S61T no effect on catalysis Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.17
-
D-fructose 1,6-bisphosphate wild-type enzyme, 30°C Escherichia coli
0.22
-
D-fructose 1,6-bisphosphate Q59A mutant, 30°C Escherichia coli
0.38
-
D-fructose 1,6-bisphosphate K325A mutant, 30°C Escherichia coli
0.43
-
D-fructose 1,6-bisphosphate S61T mutant, 30°C Escherichia coli
0.9
-
D-fructose 1,6-bisphosphate N35A mutant, 30°C Escherichia coli
2.7
-
D-fructose 1,6-bisphosphate S61A mutant, 30°C Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-fructose 1,6-bisphosphate Escherichia coli
-
glycerone phosphate + D-glyceraldehyde 3-phosphate
-
r

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Reaction

Reaction Comment Organism Reaction ID
D-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate mechanism Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-fructose 1,6-bisphosphate
-
Escherichia coli glycerone phosphate + D-glyceraldehyde 3-phosphate
-
r

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.16
-
D-fructose 1,6-bisphosphate N35A mutant, 30°C Escherichia coli
0.58
-
D-fructose 1,6-bisphosphate K325A mutant, 30°C Escherichia coli
0.85
-
D-fructose 1,6-bisphosphate S61A mutant, 30°C Escherichia coli
6.08
-
D-fructose 1,6-bisphosphate K325A mutant, 30°C Escherichia coli
6.3
-
D-fructose 1,6-bisphosphate S61T mutant, 30°C Escherichia coli
8.5
-
D-fructose 1,6-bisphosphate Q59A mutant, 30°C Escherichia coli
10.5
-
D-fructose 1,6-bisphosphate wild-type enzyme, 30°C Escherichia coli