Cloned (Comment) | Organism |
---|---|
- |
Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
K325A | decreased kcat | Escherichia coli |
N35A | 1.56% activity of the wild-type enzyme | Escherichia coli |
Q59A | no significant effect on enzyme function | Escherichia coli |
S61A | increased Km for fructose 1,6-bisphosphate | Escherichia coli |
S61T | no effect on catalysis | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.17 | - |
D-fructose 1,6-bisphosphate | wild-type enzyme, 30°C | Escherichia coli | |
0.22 | - |
D-fructose 1,6-bisphosphate | Q59A mutant, 30°C | Escherichia coli | |
0.38 | - |
D-fructose 1,6-bisphosphate | K325A mutant, 30°C | Escherichia coli | |
0.43 | - |
D-fructose 1,6-bisphosphate | S61T mutant, 30°C | Escherichia coli | |
0.9 | - |
D-fructose 1,6-bisphosphate | N35A mutant, 30°C | Escherichia coli | |
2.7 | - |
D-fructose 1,6-bisphosphate | S61A mutant, 30°C | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-fructose 1,6-bisphosphate | Escherichia coli | - |
glycerone phosphate + D-glyceraldehyde 3-phosphate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
D-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate | mechanism | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-fructose 1,6-bisphosphate | - |
Escherichia coli | glycerone phosphate + D-glyceraldehyde 3-phosphate | - |
r |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.16 | - |
D-fructose 1,6-bisphosphate | N35A mutant, 30°C | Escherichia coli | |
0.58 | - |
D-fructose 1,6-bisphosphate | K325A mutant, 30°C | Escherichia coli | |
0.85 | - |
D-fructose 1,6-bisphosphate | S61A mutant, 30°C | Escherichia coli | |
6.08 | - |
D-fructose 1,6-bisphosphate | K325A mutant, 30°C | Escherichia coli | |
6.3 | - |
D-fructose 1,6-bisphosphate | S61T mutant, 30°C | Escherichia coli | |
8.5 | - |
D-fructose 1,6-bisphosphate | Q59A mutant, 30°C | Escherichia coli | |
10.5 | - |
D-fructose 1,6-bisphosphate | wild-type enzyme, 30°C | Escherichia coli |