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Literature summary for 4.1.2.13 extracted from

  • Esposito, G.; Vitagliano, L.; Santamaria, R.; Viola, A.; Zagari, A.; Salvatore, F.
    Structural and functional analysis of aldolase B mutants related to hereditary fructose intolerance (2002), FEBS Lett., 531, 152-156.
    View publication on PubMed
Search for more literature for 4.1.2.13

Application

Application Comment Organism
medicine aldolase B deficiency causes hereditary fructose intolerance Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
-
 Homo sapiens

Protein Variants

Protein Variants Comment Organism
A149P no catalytic activity Homo sapiens
L256P melting temperature is 40°C versus 47°C for the wild-type enzyme Homo sapiens
N334K increased Km value Homo sapiens
Q354E kinetis resemble that of the wild-type enzyme Homo sapiens
W147R melting temperature is 40°C versus 47°C for the wild-type enzyme Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0017
-
 D-Fructose 1-phosphate W147R mutant, 22°C Homo sapiens
0.00173
-
 D-Fructose 1-phosphate L256P mutant, 22°C Homo sapiens
0.0022
-
 D-Fructose 1-phosphate wild-type enzyme, 22°C Homo sapiens
0.0023
-
 D-fructose 1,6-bisphosphate wild-type enzyme, 22°C Homo sapiens
0.0055
-
 D-fructose 1,6-bisphosphate W147R mutant, 22°C Homo sapiens
0.0073
-
 D-fructose 1,6-bisphosphate L256P mutant, 22°C Homo sapiens
0.0091
-
 D-fructose 1,6-bisphosphate Q354E mutant, 22°C Homo sapiens
0.022
-
 D-Fructose 1-phosphate N334K mutant, 22°C Homo sapiens
0.032
-
 D-fructose 1,6-bisphosphate N334K mutant, 22°C Homo sapiens
0.05
-
 D-Fructose 1-phosphate Q354E mutant, 22°C Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
170000
-
 tetrameric form, gel filtration Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-fructose 1,6-bisphosphate Homo sapiens
-
 glycerone phosphate + D-glyceraldehyde 3-phosphate
-
 r

Organism

Organism UniProt Comment Textmining
Homo sapiens P05062
-
-

Purification (Commentary)

Purification (Comment) Organism
95% homogeneity Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.13
-
 N334K mutant, D-fructose 1-phosphate as substrate Homo sapiens
0.28
-
 N334K mutant, D-fructose 1,6-bisphosphate as substrate Homo sapiens
0.38
-
 W147R mutant, D-fructose 1-phosphate as substrate Homo sapiens
0.58
-
 L256P mutant, D-fructose 1-phosphate as substrate Homo sapiens
0.64
-
 W147R mutant, D-fructose 1,6-bisphosphate as substrate Homo sapiens
0.81
-
 L256P mutant, D-fructose 1,6-bisphosphate as substrate Homo sapiens
0.86
-
 Q354E mutant, D-fructose 1,6-bisphosphate as substrate Homo sapiens
0.88
-
 Q354E mutant, D-fructose 1-phosphate as substrate Homo sapiens
0.94
-
 wild-type enzyme, D-fructose 1-phosphate as substrate Homo sapiens
0.97
-
 wild-type enzyme, D-fructose 1,6-bisphosphate as substrate Homo sapiens

Storage Stability

Storage Stability Organism
-20°C, wild-type and Q354E mutant: stable in 50% glycerol for 1 month, W147R and L256P mutant enzyme: aggregate after 15 days suggesting structural instability Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-fructose 1,6-bisphosphate
-
 Homo sapiens glycerone phosphate + D-glyceraldehyde 3-phosphate
-
 r
D-Fructose 1-phosphate
-
 Homo sapiens Glycerone phosphate + D-glyceraldehyde
-
 ?

Subunits

Subunits Comment Organism
More A149P mutant shows tetrameric and monomeric forms and other species with molecular masses intermediate between those corresponding to the tetramer and monomer Homo sapiens
tetramer
-
 Homo sapiens

Synonyms

Synonyms Comment Organism
aldolase B
-
 Homo sapiens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 W147R melting temperature of W147R mutant and L256P mutant is 40°C versus 47°C for the wild-type enzyme Homo sapiens
47
-
 melting temperature of the wild-type enzyme Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.059
-
 D-Fructose 1-phosphate Q354E mutant, 22°C Homo sapiens
0.085
-
 D-Fructose 1-phosphate N334K mutant, 22°C Homo sapiens
0.19
-
 D-fructose 1,6-bisphosphate N334K mutant, 22°C Homo sapiens
0.25
-
 D-Fructose 1-phosphate W147R mutant, 22°C Homo sapiens
0.38
-
 D-Fructose 1-phosphate L256P mutant, 22°C Homo sapiens
0.43
-
 D-fructose 1,6-bisphosphate W147R mutant, 22°C Homo sapiens
0.54
-
 D-fructose 1,6-bisphosphate L256P mutant, 22°C Homo sapiens
0.57
-
 D-fructose 1,6-bisphosphate Q354E mutant, 22°C Homo sapiens
0.61
-
 D-fructose 1,6-bisphosphate wild-type enzyme, 22°C Homo sapiens
0.67
-
 D-Fructose 1-phosphate wild-type enzyme, 22°C Homo sapiens
6.08
-
 D-fructose 1,6-bisphosphate L256P mutant, 22°C Homo sapiens
6.08
-
 D-fructose 1,6-bisphosphate Q354E mutant, 22°C Homo sapiens
6.08
-
 D-fructose 1,6-bisphosphate wild-type enzyme, 22°C Homo sapiens
6.08
-
 D-Fructose 1-phosphate wild-type enzyme, 22°C Homo sapiens