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Literature summary for 4.1.2.13 extracted from

  • Maurady, A.; Zdanov, A.; De Moissac, D.; Beaudry, D.; Sygusch, J.
    A conserved glutamate residue exhibits multifunctional catalytic roles in D-fructose-1,6-bisphosphate aldolases (2002), J. Biol. Chem., 277, 9474-9483.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
-
Oryctolagus cuniculus

Crystallization (Commentary)

Crystallization (Comment) Organism
vapor diffusion method and/or batch crystallization Oryctolagus cuniculus

Protein Variants

Protein Variants Comment Organism
E187A mutation compromises enzyme activity Oryctolagus cuniculus
E187Q mutation compromises enzyme activity Oryctolagus cuniculus
E189A almost no loss in catalytic activity Oryctolagus cuniculus
E189Q only small loss in catalytic activity Oryctolagus cuniculus
K229M residual background activity Oryctolagus cuniculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.1
-
D-fructose 1,6-bisphosphate native recombinant enzyme, pH 7.6, 22°C Oryctolagus cuniculus
5.7
-
D-fructose 1,6-bisphosphate E189A mutant, pH 7.6, 22°C Oryctolagus cuniculus
6.2
-
D-fructose 1,6-bisphosphate E187A mutant, pH 7.6, 22°C Oryctolagus cuniculus
10.9
-
D-fructose 1,6-bisphosphate E189Q mutant, pH 7.6, 22°C Oryctolagus cuniculus
13.4
-
D-fructose 1,6-bisphosphate E187Q mutant, pH 7.6, 22°C Oryctolagus cuniculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-fructose 1,6-bisphosphate Oryctolagus cuniculus
-
glycerone phosphate + D-glyceraldehyde 3-phosphate
-
r

Organism

Organism UniProt Comment Textmining
Oryctolagus cuniculus P00883 rabbit
-

Source Tissue

Source Tissue Comment Organism Textmining
muscle
-
Oryctolagus cuniculus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-fructose 1,6-bisphosphate
-
Oryctolagus cuniculus glycerone phosphate + D-glyceraldehyde 3-phosphate
-
r

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.03
-
D-fructose 1,6-bisphosphate E187Q mutant, pH 7.6, 22°C Oryctolagus cuniculus
0.12
-
D-fructose 1,6-bisphosphate E187A mutant, pH 7.6, 22°C Oryctolagus cuniculus
1.69
-
D-fructose 1,6-bisphosphate E189Q mutant, pH 7.6, 22°C Oryctolagus cuniculus
2.94
-
D-fructose 1,6-bisphosphate E189Q mutant, pH 7.6, 22°C Oryctolagus cuniculus
3.4
-
D-fructose 1,6-bisphosphate E189A mutant, pH 7.6, 22°C Oryctolagus cuniculus
36.9
-
D-fructose 1,6-bisphosphate E189A mutant, pH 7.6, 22°C Oryctolagus cuniculus
42.2
-
D-fructose 1,6-bisphosphate native recombinant enzyme, pH 7.6, 22°C Oryctolagus cuniculus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
E187A and E189Q mutant Oryctolagus cuniculus
8
-
broad optimum, wild-type and E189A mutant Oryctolagus cuniculus