Cloned (Comment) | Organism |
---|---|
- |
Oryctolagus cuniculus |
Crystallization (Comment) | Organism |
---|---|
vapor diffusion method and/or batch crystallization | Oryctolagus cuniculus |
Protein Variants | Comment | Organism |
---|---|---|
E187A | mutation compromises enzyme activity | Oryctolagus cuniculus |
E187Q | mutation compromises enzyme activity | Oryctolagus cuniculus |
E189A | almost no loss in catalytic activity | Oryctolagus cuniculus |
E189Q | only small loss in catalytic activity | Oryctolagus cuniculus |
K229M | residual background activity | Oryctolagus cuniculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
5.1 | - |
D-fructose 1,6-bisphosphate | native recombinant enzyme, pH 7.6, 22°C | Oryctolagus cuniculus | |
5.7 | - |
D-fructose 1,6-bisphosphate | E189A mutant, pH 7.6, 22°C | Oryctolagus cuniculus | |
6.2 | - |
D-fructose 1,6-bisphosphate | E187A mutant, pH 7.6, 22°C | Oryctolagus cuniculus | |
10.9 | - |
D-fructose 1,6-bisphosphate | E189Q mutant, pH 7.6, 22°C | Oryctolagus cuniculus | |
13.4 | - |
D-fructose 1,6-bisphosphate | E187Q mutant, pH 7.6, 22°C | Oryctolagus cuniculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-fructose 1,6-bisphosphate | Oryctolagus cuniculus | - |
glycerone phosphate + D-glyceraldehyde 3-phosphate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oryctolagus cuniculus | P00883 | rabbit | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
muscle | - |
Oryctolagus cuniculus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-fructose 1,6-bisphosphate | - |
Oryctolagus cuniculus | glycerone phosphate + D-glyceraldehyde 3-phosphate | - |
r |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.03 | - |
D-fructose 1,6-bisphosphate | E187Q mutant, pH 7.6, 22°C | Oryctolagus cuniculus | |
0.12 | - |
D-fructose 1,6-bisphosphate | E187A mutant, pH 7.6, 22°C | Oryctolagus cuniculus | |
1.69 | - |
D-fructose 1,6-bisphosphate | E189Q mutant, pH 7.6, 22°C | Oryctolagus cuniculus | |
2.94 | - |
D-fructose 1,6-bisphosphate | E189Q mutant, pH 7.6, 22°C | Oryctolagus cuniculus | |
3.4 | - |
D-fructose 1,6-bisphosphate | E189A mutant, pH 7.6, 22°C | Oryctolagus cuniculus | |
36.9 | - |
D-fructose 1,6-bisphosphate | E189A mutant, pH 7.6, 22°C | Oryctolagus cuniculus | |
42.2 | - |
D-fructose 1,6-bisphosphate | native recombinant enzyme, pH 7.6, 22°C | Oryctolagus cuniculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
E187A and E189Q mutant | Oryctolagus cuniculus |
8 | - |
broad optimum, wild-type and E189A mutant | Oryctolagus cuniculus |