Cloned (Comment) | Organism |
---|---|
- |
Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
E174A | severely crippled catalysis | Escherichia coli |
E181A | mutant enzyme has wild-type properties | Escherichia coli |
E182A | decrease in kcat | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
D-fructose 1,6-bisphosphate | E182A mutant, 30°C | Escherichia coli | |
0.13 | - |
D-fructose 1,6-bisphosphate | E174A mutant, 30°C | Escherichia coli | |
0.17 | - |
D-fructose 1,6-bisphosphate | wild-type enzyme, 30°C | Escherichia coli | |
0.3 | - |
D-fructose 1,6-bisphosphate | E181A mutant, 30°C | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
class II enzyme | Escherichia coli |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
D-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate | mechanism | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-fructose 1,6-bisphosphate | - |
Escherichia coli | glycerone phosphate + D-glyceraldehyde 3-phosphate | - |
r |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.013 | - |
D-fructose 1,6-bisphosphate | E174A mutant, 30°C | Escherichia coli | |
0.033 | - |
D-fructose 1,6-bisphosphate | E182A mutant, 30°C | Escherichia coli | |
5.8 | - |
D-fructose 1,6-bisphosphate | E181A mutant, 30°C | Escherichia coli | |
10.5 | - |
D-fructose 1,6-bisphosphate | wild-type enzyme, 30°C | Escherichia coli |