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Literature summary for 4.1.2.13 extracted from

  • Esposito, G.; Vitagliano, L.; Costanzo, P.; Borrelli, L.; Barone, R.; Pavone, L.; Izzo, P.; Zagari, A.; Salvatore, F.
    Human aldolase A natural mutants: relationship between flexibility of the C-terminal region and enzyme function (2004), Biochem. J., 380, 51-56.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.1.2.13

Cloned(Commentary)

Cloned (Comment) Organism
wild-type enzyme and mutant enzymes G346S and E206K, expression in Escherichia coli Homo sapiens

Protein Variants

Protein Variants Comment Organism
E206K mutant has a KM-value 2fold higher than that of both the Gly346S mutant and the wild-type enzyme, and a turnover-number value 40% less than the wild-type Homo sapiens
G346S mutant enzyme has the same KM-value as the wild-type enzyme, but a 4fold lower turnover-number Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0519
-
 D-fructose 1,6-bisphosphate wild-type enzyme Homo sapiens
0.0566
-
 D-fructose 1,6-bisphosphate mutant enzyme G346S Homo sapiens
0.105
-
 D-fructose 1,6-bisphosphate mutant enzyme E206K Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
170000
-
 gel filtration Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens P04075
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant wild-type and mutant enzymes G346S and E206K Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.1
-
 mutant enzyme G346S Homo sapiens
15.7
-
 mutant enzyme E206K Homo sapiens
22.3
-
 wild-type enzyme Homo sapiens

Storage Stability

Storage Stability Organism
-20°C, 50% v/v glycerol/20 mM TrisHCl, stable for at least 1 month Homo sapiens
4°C, 20 mM Tris/HCl, pH 7.4, stable for at least 2 weeks Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-fructose 1,6-bisphosphate
-
 Homo sapiens glycerone phosphate + D-glyceraldehyde 3-phosphate
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
48
-
 melting temperature of mutant E206K Homo sapiens
54
-
 melting temperature of wild-type enzyme is 54.4°C Homo sapiens
55
-
 melting temperature of mutant enzyme G346S is 54.8°C Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.7
-
 D-fructose 1,6-bisphosphate mutant enzyme G346S Homo sapiens
10.7
-
 D-fructose 1,6-bisphosphate mutant enzyme E206K Homo sapiens
16.7
-
 D-fructose 1,6-bisphosphate wild-type enzyme Homo sapiens