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Literature summary for 4.1.2.13 extracted from
- Fructose 1,6-bisphosphate aldolase/phosphatase may be an ancestral gluconeogenic enzyme (2010), Nature, 464, 1077-1081.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | AMP, ADP or glucose (2 mM) have no effect on activity | Pyrobaculum neutrophilum | |
| additional information | AMP, ADP or glucose (2 mM) have no effect on activity | Cenarchaeum symbiosum | |
| additional information | AMP, ADP or glucose (2 mM) have no effect on activity | Metallosphaera sedula | |
| additional information | AMP, ADP or glucose (2 mM) have no effect on activity | Ignicoccus hospitalis |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli | Pyrobaculum neutrophilum |
| expressed in Escherichia coli | Cenarchaeum symbiosum |
| expressed in Escherichia coli | Ignicoccus hospitalis |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| K232R | the mutant protein has lost FBP aldolase activity, whereas the FBP phosphatase activity is 3fold enhanced | Cenarchaeum symbiosum |
| Y348F | the mutant shows unaltered FBP aldolase activity, but has lost FBP phosphatase activity (less than 5%) | Cenarchaeum symbiosum |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Borohydride | - |
Cenarchaeum symbiosum |  |
| Borohydride | - |
Ignicoccus hospitalis | |
| Borohydride | - |
Metallosphaera sedula | |
| Borohydride | - |
Pyrobaculum neutrophilum | |
| EDTA | - |
Cenarchaeum symbiosum | |
| EDTA | - |
Ignicoccus hospitalis | |
| EDTA | - |
Metallosphaera sedula | |
| EDTA | - |
Pyrobaculum neutrophilum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.1 | - |
D-fructose 1,6-bisphosphate | at 48°C | Cenarchaeum symbiosum | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | the enzyme contains four Mg2+ ions | Pyrobaculum neutrophilum | |
| Mg2+ | the enzyme contains four Mg2+ ions | Cenarchaeum symbiosum | |
| Mg2+ | the enzyme contains four Mg2+ ions | Metallosphaera sedula | |
| Mg2+ | the enzyme contains four Mg2+ ions | Ignicoccus hospitalis | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cenarchaeum symbiosum | - |
- |
- |
| Ignicoccus hospitalis | - |
- |
- |
| Metallosphaera sedula | - |
- |
- |
| Pyrobaculum neutrophilum | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| MonoQ-Sepharose column chromatography and gel filtration | Ignicoccus hospitalis |
| Ni2+ affinity chromatography | Cenarchaeum symbiosum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-fructose 1,6-bisphosphate | - |
Pyrobaculum neutrophilum | glycerone phosphate + D-glyceraldehyde 3-phosphate | - |
? | |
| D-fructose 1,6-bisphosphate | - |
Cenarchaeum symbiosum | glycerone phosphate + D-glyceraldehyde 3-phosphate | - |
? | |
| D-fructose 1,6-bisphosphate | - |
Metallosphaera sedula | glycerone phosphate + D-glyceraldehyde 3-phosphate | - |
? | |
| D-fructose 1,6-bisphosphate | - |
Ignicoccus hospitalis | glycerone phosphate + D-glyceraldehyde 3-phosphate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| FBP aldolase/phosphatase | - |
Pyrobaculum neutrophilum |
| FBP aldolase/phosphatase | - |
Cenarchaeum symbiosum |
| FBP aldolase/phosphatase | - |
Metallosphaera sedula |
| FBP aldolase/phosphatase | - |
Ignicoccus hospitalis |
| fructose 1,6-bisphosphate aldolase/phosphatase | bifunctional enzyme with both fructose 1,6-bisphosphate aldolase and fructose 1,6-bisphosphate phosphatase activity | Pyrobaculum neutrophilum |
| fructose 1,6-bisphosphate aldolase/phosphatase | bifunctional enzyme with both fructose 1,6-bisphosphate aldolase and fructose 1,6-bisphosphate phosphatase activity | Cenarchaeum symbiosum |
| fructose 1,6-bisphosphate aldolase/phosphatase | bifunctional enzyme with both fructose 1,6-bisphosphate aldolase and fructose 1,6-bisphosphate phosphatase activity | Metallosphaera sedula |
| fructose 1,6-bisphosphate aldolase/phosphatase | bifunctional enzyme with both fructose 1,6-bisphosphate aldolase and fructose 1,6-bisphosphate phosphatase activity | Ignicoccus hospitalis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 100 | - |
the enzyme survives boiling for 1 h | Ignicoccus hospitalis |
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