Activating Compound | Comment | Organism | Structure |
---|---|---|---|
cysteine | highest activity at 1 mM cysteine | Haloferax volcanii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | 2 mM, complete inhibition of activity, which could be reversed by the addition of Mn2+ (3 mM) | Haloferax volcanii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.24 | - |
D-fructose 1,6-bisphosphate | pH 7.5, 42°C | Haloferax volcanii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
FeSO4 | the enzyme requires divalent cation for activity. The highest activity is obtained with MnCl2 (100%, 50 U/mg), which can be partially replaced by FeSO4 (40% residual activity) | Haloferax volcanii | |
KCl | highest activity at 1 mM KCl | Haloferax volcanii | |
MnCl2 | the enzyme requires divalent cation for activity. The highest activity is obtained with MnCl2 (100%, 50 U/mg), which can be partially replaced by FeSO4 (40% residual activity) | Haloferax volcanii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
42000 | - |
2 * 42000, SDS-PAGE | Haloferax volcanii |
80000 | - |
- |
Haloferax volcanii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-fructose 1,6-bisphosphate | Haloferax volcanii | the enzyme has an anabolic function catalyzing fructose-1,6-bisphosphate formation in the course of gluconeogenesis | glycerone phosphate + D-glyceraldehyde 3-phosphate | - |
r | |
D-fructose 1,6-bisphosphate | Haloferax volcanii DSM 3757 | the enzyme has an anabolic function catalyzing fructose-1,6-bisphosphate formation in the course of gluconeogenesis | glycerone phosphate + D-glyceraldehyde 3-phosphate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Haloferax volcanii | D4GYE0 | - |
- |
Haloferax volcanii DSM 3757 | D4GYE0 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-fructose 1,6-bisphosphate | - |
Haloferax volcanii | glycerone phosphate + D-glyceraldehyde 3-phosphate | - |
r | |
D-fructose 1,6-bisphosphate | the enzyme has an anabolic function catalyzing fructose-1,6-bisphosphate formation in the course of gluconeogenesis | Haloferax volcanii | glycerone phosphate + D-glyceraldehyde 3-phosphate | - |
r | |
D-fructose 1,6-bisphosphate | - |
Haloferax volcanii DSM 3757 | glycerone phosphate + D-glyceraldehyde 3-phosphate | - |
r | |
D-fructose 1,6-bisphosphate | the enzyme has an anabolic function catalyzing fructose-1,6-bisphosphate formation in the course of gluconeogenesis | Haloferax volcanii DSM 3757 | glycerone phosphate + D-glyceraldehyde 3-phosphate | - |
r |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 42000, SDS-PAGE | Haloferax volcanii |
Synonyms | Comment | Organism |
---|---|---|
FBA | - |
Haloferax volcanii |
fructose-1,6-bisphosphate aldolase | - |
Haloferax volcanii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
42 | - |
assay at | Haloferax volcanii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Haloferax volcanii |
Organism | Comment | Expression |
---|---|---|
Haloferax volcanii | highly increased transcript levels of fba in fructose-grown cells compared to those in glucose-grown cells, indicating fructosespecific upregulation | up |
General Information | Comment | Organism |
---|---|---|
malfunction | deletion mutant loses the ability to grow on fructose, but growth on glucose is not inhibited and is even slightly stimulated | Haloferax volcanii |
physiological function | the enzyme has an anabolic function catalyzing fructose-1,6-bisphosphate formation in the course of gluconeogenesis. The enzyme is essential for growth on fructose | Haloferax volcanii |