Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Metroxylon sagu |
expression in Escherichia coli BL21 (DE3) | Metroxylon sagu |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | - |
Metroxylon sagu | 9507 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
39000 | - |
non-denaturing SDS-PAGE | Metroxylon sagu |
160000 | - |
gel filtration | Metroxylon sagu |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-fructose 1,6-bisphosphate | Metroxylon sagu | - |
glycerone phosphate + D-glyceraldehyde 3-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Metroxylon sagu | - |
- |
- |
Metroxylon sagu | - |
sago palm | - |
Purification (Comment) | Organism |
---|---|
- |
Metroxylon sagu |
Ni-NTA column chromatography | Metroxylon sagu |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Metroxylon sagu | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-fructose 1,6-bisphosphate | - |
Metroxylon sagu | glycerone phosphate + D-glyceraldehyde 3-phosphate | - |
? | |
D-fructose 1,6-bisphosphate | - |
Metroxylon sagu | glycerone phosphate + D-glyceraldehyde 3-phosphate | - |
r | |
Glycerone phosphate + D-glyceraldehyde 3-phosphate | - |
Metroxylon sagu | D-Fructose 1,6-bisphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 39000, SDS-PAGE | Metroxylon sagu |
homotetramer | 4 * 39140, calculated from amino acid sequence | Metroxylon sagu |
tetramer | the modeled structure is a homotetramer containing an alpha/beta-TIM-barrel at the center | Metroxylon sagu |
Synonyms | Comment | Organism |
---|---|---|
FBAld | - |
Metroxylon sagu |
Fructose 1,6-bisphosphate aldolase | - |
Metroxylon sagu |
fructose-1,6-bisphosphate aldolase | - |
Metroxylon sagu |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Metroxylon sagu | calculated from amino acid sequence | - |
6.5 |
General Information | Comment | Organism |
---|---|---|
physiological function | overproduction of the recombinant enzyme (msFBAld) in Escherichia coli results in increased tolerance towards salinity | Metroxylon sagu |
physiological function | the enzyme plays vital role in glycolysis and gluconeogenesis | Metroxylon sagu |