Literature summary for 4.1.2.14 extracted from

Sutter, J.M.; Taestensen, J.B.; Johnsen, U.; Soppa, J.; Schoenheit, P.
Key enzymes of the semiphosphorylative Entner-Doudoroff pathway in the haloarchaeon Haloferax volcanii characterization of glucose dehydrogenase, gluconate dehydratase, and 2-keto-3-deoxy-6-phosphogluconate aldolase (2016), J. Bacteriol., 198, 2251-2262 .

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli and Haloferax volcanii H1209	Haloferax volcanii

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.29	-	2-dehydro-3-deoxy-6-phospho-D-gluconate	at pH 7.0 and 42°C	Haloferax volcanii
38	-	D-glyceraldehyde 3-phosphate	at pH 7.0 and 42°C	Haloferax volcanii

Metals/Ions

Metals/Ions	Comment	Organism	Structure
K+	maximal activities at concentrations of between 1 and 1.5 M KCl	Haloferax volcanii

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
267000	-	gel filtration	Haloferax volcanii

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
2-dehydro-3-deoxy-6-phospho-D-gluconate	Haloferax volcanii	-	pyruvate + D-glyceraldehyde 3-phosphate	-	r
2-dehydro-3-deoxy-6-phospho-D-gluconate	Haloferax volcanii H26	-	pyruvate + D-glyceraldehyde 3-phosphate	-	r

Organism

Organism	UniProt	Comment	Textmining
Haloferax volcanii	D4GV57	-	-
Haloferax volcanii H26	D4GV57	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni-NTA column chromatography and Superdex 200 gel filtration	Haloferax volcanii

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2-dehydro-3-deoxy-6-phospho-D-galactonate	low activity	Haloferax volcanii	?	-	?
2-dehydro-3-deoxy-6-phospho-D-galactonate	low activity	Haloferax volcanii H26	?	-	?
2-dehydro-3-deoxy-6-phospho-D-gluconate	-	Haloferax volcanii	pyruvate + D-glyceraldehyde 3-phosphate	-	r
2-dehydro-3-deoxy-6-phospho-D-gluconate	100% activity	Haloferax volcanii	pyruvate + D-glyceraldehyde 3-phosphate	-	r
2-dehydro-3-deoxy-6-phospho-D-gluconate	-	Haloferax volcanii H26	pyruvate + D-glyceraldehyde 3-phosphate	-	r
2-dehydro-3-deoxy-6-phospho-D-gluconate	100% activity	Haloferax volcanii H26	pyruvate + D-glyceraldehyde 3-phosphate	-	r
2-dehydro-3-deoxy-D-gluconate	about 1% activity compared to 2-dehydro-3-deoxy-6-phospho-D-gluconate	Haloferax volcanii	pyruvate + D-glyceraldehyde	-	?
2-dehydro-3-deoxy-D-gluconate	about 1% activity compared to 2-dehydro-3-deoxy-6-phospho-D-gluconate	Haloferax volcanii H26	pyruvate + D-glyceraldehyde	-	?
pyruvate + D-glyceraldehyde 3-phosphate	-	Haloferax volcanii	2-dehydro-3-deoxy-6-phospho-D-gluconate	-	r
pyruvate + D-glyceraldehyde 3-phosphate	-	Haloferax volcanii H26	2-dehydro-3-deoxy-6-phospho-D-gluconate	-	r

Subunits

Subunits	Comment	Organism
homododecamer	12 * 22400, calculated from amino acid sequence	Haloferax volcanii

Synonyms

Synonyms	Comment	Organism
2-keto-3-deoxy-6-phosphogluconate aldolase	-	Haloferax volcanii
KDPG aldolase	-	Haloferax volcanii
KDPGA	-	Haloferax volcanii

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7	-	-	Haloferax volcanii

pH Range

pH Minimum	pH Maximum	Comment	Organism
5.5	9	50% activity between pH 5.5 and 9.0	Haloferax volcanii

General Information

General Information	Comment	Organism
metabolism	the enzyme is essential for glucose degradation	Haloferax volcanii

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Release 2023.1 (January 2023)