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Literature summary for 4.1.2.17 extracted from
- Kinetic modelling of aldolase-catalyzed addition between dihydroxyacetone phosphate and (S)-alaninal (2008), Biochem. Eng. J., 41, 95-103.
No PubMed abstract available
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli XL1 blue MRF cells | Escherichia coli |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| (S)-Cbz-alaninal | non-competitive inhibition | Escherichia coli |  |
| methylglyoxal | competitive inhibition | Escherichia coli | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 54.7 | - |
dihydroxyacetone phosphate | - |
Escherichia coli | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 24000 | - |
monomer | Escherichia coli |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dihydroxyacetone phosphate + D-glyceraldehyde 3-phosphate | Escherichia coli | - |
? | - |
r | |
| dihydroxyacetone phosphate + L-lactaldehyde | Escherichia coli | - |
L-fuculose 1-phosphate | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| by metal-chelate affinity chromatography and (NH4)2SO4 precipitation after ZnSO4 incubation | Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 10 | - |
- |
Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (S)-Cbz-alaninal + dihydroxyacetone phosphate | - |
Escherichia coli | (3R,4R,5S)-5-(benzyloxycarbonylamino)-5,6-dideoxy-1-phosphonohex-2-ulose | - |
? | |
| dihydroxyacetone phosphate | - |
Escherichia coli | methylglyoxal + phosphate | - |
? | |
| dihydroxyacetone phosphate + D-glyceraldehyde 3-phosphate | - |
Escherichia coli | ? | - |
r | |
| dihydroxyacetone phosphate + L-lactaldehyde | - |
Escherichia coli | L-fuculose 1-phosphate | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | - |
Escherichia coli |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| FucA | - |
Escherichia coli |
| fuculose-1-phosphate aldolase | - |
Escherichia coli |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
FucA activity assay | Escherichia coli |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.36 | - |
dihydroxyacetone phosphate | - |
Escherichia coli | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
FucA activity assay | Escherichia coli |
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Release 2023.1 (January 2023)
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