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Literature summary for 4.1.2.19 extracted from
- Other deoxy sugar aldolases (1972), The Enzymes, 3rd Ed. (Boyer, P. D. , ed. ), 7, 303-321.
No PubMed abstract available
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | the enzyme contains 12 sulfhydryl groups per molecule | Escherichia coli |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1,10-phenanthroline | competitive with L-rhamnulose 1-phosphate or glycerone phosphate; noncompetitive with L-lactaldehyde | Escherichia coli |  |
| 2,2'-dipyridyl | - |
Escherichia coli | |
| 8-hydroxyquinoline 5-sulfonic acid | - |
Escherichia coli | |
| EDTA | - |
Escherichia coli | |
| Li+ | - |
Escherichia coli | |
| NaBH4 | - |
Escherichia coli | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.2 | - |
glycerone phosphate | - |
Escherichia coli | |
| 0.2 | - |
L-rhamnulose 1-phosphate | - |
Escherichia coli | |
| 1 | - |
L-lactaldehyde | - |
Escherichia coli | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | restores activity of the enzyme after inactivation by dialysis against chelating agents or by treatment with SDS and 2-mercaptoethanol | Escherichia coli | |
| Cs+ | Na+, Cs+, NH4+, Rb+ and K+, in the order of increasing effectiveness markedly enhance activity | Escherichia coli | |
| K+ | Na+, Cs+, NH4+, Rb+ and K+, in the order of increasing effectiveness markedly enhance activity | Escherichia coli | |
| Mg2+ | restores activity of the enzyme after inactivation by dialysis against chelating agents or by treatment with SDS and 2-mercaptoethanol | Escherichia coli | |
| Mn2+ | restores activity of the enzyme after inactivation by dialysis against chelating agents or by treatment with SDS and 2-mercaptoethanol | Escherichia coli | |
| Na+ | Na+, Cs+, NH4+, Rb+ and K+, in the order of increasing effectiveness markedly enhance activity | Escherichia coli | |
| NH4+ | Na+, Cs+, NH4+, Rb+ and K+, in the order of increasing effectiveness markedly enhance activity | Escherichia coli | |
| Ni2+ | restores activity of the enzyme after inactivation by dialysis against chelating agents or by treatment with SDS and 2-mercaptoethanol | Escherichia coli | |
| Rb+ | Na+, Cs+, NH4+, Rb+ and K+, in the order of increasing effectiveness markedly enhance activity | Escherichia coli | |
| Zn | zinc metalloenzyme | Escherichia coli | |
| Zn | contains 2 gatom of zinc per mol of enzyme | Escherichia coli | |
| Zn2+ | restores activity of the enzyme after inactivation by dialysis against chelating agents or by treatment with SDS and 2-mercaptoethanol | Escherichia coli | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 35000 | - |
4 * 35000 | Escherichia coli |
| 130000 | 140000 | sucrose density gradient centrifugation | Escherichia coli |
| 135000 | - |
gel filtration | Escherichia coli |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
| Lactiplantibacillus plantarum | - |
- |
- |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 0-4°C, pH 7.0, 0.01 M mercaptoethanol, stable for at least 3 months | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Glycerone phosphate + (S)-lactaldehyde | - |
Escherichia coli | L-Rhamnulose 1-phosphate | - |
? | |
| Glycerone phosphate + acetaldehyde | - |
Escherichia coli | 5-Deoxy-L-xylulose 1-phosphate | - |
? | |
| glycerone phosphate + D-glyceraldehyde | - |
Escherichia coli | D-sorbose 1-phosphate | - |
? | |
| Glycerone phosphate + D-lactaldehyde | - |
Escherichia coli | 6-Deoxy-D-sorbose 1-phosphate | - |
? | |
| Glycerone phosphate + formaldehyde | - |
Escherichia coli | D-Erythrulose 1-phosphate | - |
? | |
| Glycerone phosphate + glycolaldehyde | - |
Escherichia coli | L-Xylulose 1-phosphate | - |
? | |
| L-Rhamnulose 1-phosphate | - |
Lactiplantibacillus plantarum | Glycerone phosphate + (S)-lactaldehyde | - |
? | |
| L-Rhamnulose 1-phosphate | - |
Escherichia coli | Glycerone phosphate + (S)-lactaldehyde | - |
? | |
| L-Sorbose 1-phosphate | - |
Escherichia coli | Glycerone phosphate + D-glyceraldehyde | - |
? | |
| Xylulose 1-phosphate | - |
Escherichia coli | Glycerone phosphate + glycoaldehyde | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 35000 | Escherichia coli |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 38.3 | - |
L-rhamnulose 1-phosphate | - |
Escherichia coli | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Escherichia coli |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
0-4°C, stable for at least 3 months | Escherichia coli |
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