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Literature summary for 4.1.2.22 extracted from
- Purification and properties of two fructose-6-phosphate phosphoketolase in Bifidobacterium (1976), Antonie van Leeuwenhoek, 42, 49-57.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| (NH4)2SO4 | - |
Bifidobacterium dentium |  |
| (NH4)2SO4 | - |
Bifidobacterium pseudolongum subsp. globosum | |
| hydroxylamine | - |
Bifidobacterium dentium | |
| hydroxylamine | - |
Bifidobacterium pseudolongum subsp. globosum | |
| Iodosobenzoate | - |
Bifidobacterium dentium | |
| Iodosobenzoate | - |
Bifidobacterium pseudolongum subsp. globosum | |
| p-hydroxymercuribenzoate | - |
Bifidobacterium dentium | |
| p-hydroxymercuribenzoate | - |
Bifidobacterium pseudolongum subsp. globosum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.4 | - |
fructose 6-phosphate | - |
Bifidobacterium pseudolongum subsp. globosum | |
| 39 | - |
fructose 6-phosphate | - |
Bifidobacterium dentium | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | maximal activation in presence of Mg2+ | Bifidobacterium pseudolongum subsp. globosum | |
| Mg2+ | 45% of the activation with Mn2+ | Bifidobacterium dentium | |
| Mn2+ | 44% of the activation with Mg2+ | Bifidobacterium pseudolongum subsp. globosum | |
| Mn2+ | maximal activation with Mn2+ | Bifidobacterium dentium | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 160000 | - |
gel filtration | Bifidobacterium dentium |
| 290000 | - |
gel filtration | Bifidobacterium pseudolongum subsp. globosum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bifidobacterium dentium | - |
- |
- |
| Bifidobacterium pseudolongum subsp. globosum | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Bifidobacterium pseudolongum subsp. globosum |
- |
Bifidobacterium dentium |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 24 | - |
- |
Bifidobacterium pseudolongum subsp. globosum |
| 30 | - |
- |
Bifidobacterium dentium |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-Fructose 6-phosphate + phosphate | - |
Bifidobacterium pseudolongum subsp. globosum | Acetyl phosphate + D-erythrose 4-phosphate + H2O | - |
? | |
| D-Fructose 6-phosphate + phosphate | - |
Bifidobacterium dentium | Acetyl phosphate + D-erythrose 4-phosphate + H2O | - |
? | |
| D-ribose 5-phosphate + phosphate | 13.3% of the activity with D-fructose 6-phosphate | Bifidobacterium dentium | ? | - |
? | |
| D-ribose 5-phosphate + phosphate | 25% of the activity with D-fructose 6-phosphate | Bifidobacterium pseudolongum subsp. globosum | ? | - |
? | |
| D-ribose 5-phosphate + phosphate | 208% of the activity with D-fructose 6-phosphate | Bifidobacterium pseudolongum subsp. globosum | ? | - |
? | |
| Fructose 1-6-diphosphate + phosphate | 13.3% of the activity with D-fructose 6-phosphate | Bifidobacterium dentium | ? | - |
? | |
| Fructose 1-6-diphosphate + phosphate | 20.8% of the activity with D-fructose 6-phosphate | Bifidobacterium pseudolongum subsp. globosum | ? | - |
? | |
| Sedoheptulose 7-phosphate + phosphate | 13.3% of the activity with D-fructose 6-phosphate | Bifidobacterium dentium | ? | - |
? | |
| Sedoheptulose 7-phosphate + phosphate | 16.6% of the activity with D-fructose 6-phosphate | Bifidobacterium pseudolongum subsp. globosum | ? | - |
? | |
| Xylulose 5-phosphate + phosphate | 166% of the activity with D-fructose 6-phosphate | Bifidobacterium pseudolongum subsp. globosum | ? | - |
? | |
| Xylulose 5-phosphate + phosphate | 6.6% of the activity with D-fructose 6-phosphate | Bifidobacterium dentium | ? | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 57 | - |
5 min, stable | Bifidobacterium pseudolongum subsp. globosum |
| 57 | - |
5 min, 40% loss of activity | Bifidobacterium dentium |
| 65 | - |
5 min, about 65% loss of activity | Bifidobacterium pseudolongum subsp. globosum |
| 65 | - |
5 min, about 45% loss of activity | Bifidobacterium dentium |
| 80 | - |
5 min, complete loss of activity | Bifidobacterium dentium |
| 80 | - |
5 min, about 85% loss of activity | Bifidobacterium pseudolongum subsp. globosum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 6 | - |
Bifidobacterium pseudolongum subsp. globosum |
| 7 | - |
- |
Bifidobacterium dentium |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 8 | pH 4.0: about 40% of maximal activity, pH 9.0: about 30% of maximal activity | Bifidobacterium pseudolongum subsp. globosum |
| 4 | 9 | about 30% of maximal activity at pH 4.0 and at pH 9.0 | Bifidobacterium dentium |
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