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Literature summary for 4.1.2.25 extracted from
- Folate biosynthesis in higher plants. cDNA cloning, heterologous expression, and characterization of Dihydroneopterin aldolases (2004), Plant Physiol., 135, 103-111.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli | Arabidopsis thaliana |
| expressed in Escherichia coli | Solanum lycopersicum |
| expression in Escherichia coli | Solanum lycopersicum |
| expression in Escherichia coli | Arabidopsis thaliana |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 13600 | - |
x * 14685, calculated, x * 13600, SDS-PAGE | Arabidopsis thaliana |
| 14000 | - |
8 * 14000, gel filtration | Arabidopsis thaliana |
| 14100 | - |
x * 16300, calculated, x * 14100, SDS-PAGE of truncated recombinant protein | Arabidopsis thaliana |
| 14600 | - |
8 * 14600, gel filtration | Solanum lycopersicum |
| 14600 | - |
x * 14600, SDS-PAGE | Solanum lycopersicum |
| 14685 | - |
x * 14685, calculated, x * 13600, SDS-PAGE | Arabidopsis thaliana |
| 16300 | - |
x * 16300, calculated, x * 14100, SDS-PAGE of truncated recombinant protein | Arabidopsis thaliana |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arabidopsis thaliana | - |
- |
- |
| Arabidopsis thaliana | Q9FM54 | bifunctional epimerase, EC 5.1.99.8, and aldolase | - |
| Arabidopsis thaliana | Q9SF23 | bifunctional epimerase, EC 5.1.99.8, and aldolase | - |
| Solanum lycopersicum | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| homogeneity | Arabidopsis thaliana |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Arabidopsis thaliana | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 7,8-dihydromonapterin | - |
Solanum lycopersicum | 6-hydroxymethyl-7,8-dihydropterin + glycolaldehyde | - |
? |  |
| 7,8-dihydromonapterin | - |
Arabidopsis thaliana | 6-hydroxymethyl-7,8-dihydropterin + glycolaldehyde | - |
? | |
| 7,8-dihydroneopterin | - |
Arabidopsis thaliana | 6-hydroxymethyl-7,8-dihydropterin + glycolaldehyde | - |
? | |
| 7,8-dihydroneopterin | - |
Solanum lycopersicum | 6-hydroxymethyl-7,8-dihydropterin + glycolaldehyde | - |
? | |
| additional information | enzyme also mediates the epimerisation of 7,8-dihydroneopterin to 7,8-dihydromonapterin | Arabidopsis thaliana | ? | - |
? | |
| additional information | enzyme also mediates the epimerisation of 7,8-dihydroneopterin to 7,8-dihydromonapterin | Solanum lycopersicum | ? | - |
? | |
| additional information | enzyme catalyzes both the conversion of 7,8-dihydroneopterin to 6-hydroxymethyl-7,8-dihydropterin, reaction of EC 4.1.2.25, and the epimerization of 7,8-dihydroneopterin to 7,8-dihydromonapterin, it carries out the aldol cleavage reaction on the epimerization product, 7,8-dihydromonapterin, as well as on 7,8-dihydroneopterin | Solanum lycopersicum | ? | - |
? | |
| additional information | enzyme catalyzes both the conversion of 7,8-dihydroneopterin to 6-hydroxymethyl-7,8-dihydropterin, reaction of EC 4.1.2.25, and the epimerization of 7,8-dihydroneopterin to 7,8-dihydromonapterin, it carries out the aldol cleavage reaction on the epimerization product, 7,8-dihydromonapterin, as well as on 7,8-dihydroneopterin | Arabidopsis thaliana | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 14600, SDS-PAGE | Solanum lycopersicum |
| ? | x * 14685, calculated, x * 13600, SDS-PAGE | Arabidopsis thaliana |
| ? | x * 16300, calculated, x * 14100, SDS-PAGE of truncated recombinant protein | Arabidopsis thaliana |
| octamer | 8 * 14000, gel filtration | Arabidopsis thaliana |
| octamer | 8 * 14600, gel filtration | Solanum lycopersicum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| At3g11750 | - |
Arabidopsis thaliana |
| At5g62980 | - |
Arabidopsis thaliana |
| FolB1 | - |
Solanum lycopersicum |
| FolB1 | - |
Arabidopsis thaliana |
| FolB2 | - |
Arabidopsis thaliana |
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