KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00016 | - |
7,8-dihydroneopterin | pH 7.0, 22°C | Mycobacterium tuberculosis | |
0.00017 | - |
7,8-dihydromonapterin | pH 7.0, 22°C | Mycobacterium tuberculosis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | no metal required | Mycobacterium tuberculosis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
16585 | - |
apo-enzyme, 4 * 16585, and octamer for 7,8-dihydroxanthopterin-bound form, 8 * 16585, mass spectrometry | Mycobacterium tuberculosis |
62300 | - |
apo-enzyme, mass spectrometry | Mycobacterium tuberculosis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | P9WNC5 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
6-hydroxymethyl-7,8-dihydropterin + glycolaldehyde | - |
Mycobacterium tuberculosis | 7,8-dihydroneopterin + 7,8-dihydromonapterin | reaction is reversible, and C-C bond formation has both 7,8-dihydroneopterin and 7,8-dihydromonapterin as products plus 7,8-dihydroxanthopterin | r | |
7,8-dihydromonapterin | - |
Mycobacterium tuberculosis | 6-hydroxymethyl-7,8-dihydropterin + glycolaldehyde | - |
r | |
7,8-dihydroneopterin | - |
Mycobacterium tuberculosis | 6-hydroxymethyl-7,8-dihydropterin + glycolaldehyde | - |
r | |
additional information | enzyme can utilize both dihydroneopterin and 7,8-dihydromonapterin as substrates to generate 6-hydroxymethyl-7,8-dihydropterin. The reaction generates three different pterin products, one of which is not produced by other wild-type dihydroneopterin aldolases. The enzyme-substrate complex partitions 51% in the first turnover to form the aldolase products, 24% to the epimerase product and 25% to the oxygenase products. The aldolase reaction is strongly pH dependent. Chemistry is rate limiting for the aldolase reaction, and two protons and a likely solvent contribution are involved in formation and breakage of a common intermediate | Mycobacterium tuberculosis | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | apo-enzyme, 4 * 16585, and octamer for 7,8-dihydroxanthopterin-bound form, 8 * 16585, mass spectrometry | Mycobacterium tuberculosis |
Synonyms | Comment | Organism |
---|---|---|
DHNA | - |
Mycobacterium tuberculosis |
FolB | - |
Mycobacterium tuberculosis |
Rv3607c | - |
Mycobacterium tuberculosis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0054 | - |
7,8-dihydroneopterin | pH 7.0, 22°C | Mycobacterium tuberculosis | |
0.006 | - |
7,8-dihydromonapterin | pH 7.0, 22°C | Mycobacterium tuberculosis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
36 | - |
7,8-dihydroneopterin | pH 7.0, 22°C | Mycobacterium tuberculosis | |
38 | - |
7,8-dihydromonapterin | pH 7.0, 22°C | Mycobacterium tuberculosis |