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Literature summary for 4.1.2.25 extracted from

  • Wang, Y.; Xu, H.; Grochowski, L.L.; White, R.H.
    Biochemical characterization of a dihydroneopterin aldolase used for methanopterin biosynthesis in methanogens (2014), J. Bacteriol., 196, 3191-3198 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.1.2.25

Protein Variants

Protein Variants Comment Organism
E25Q the mutation causes a more than 25fold increase in Km value Methanocaldococcus jannaschii
H35N the mutant shows an about 20fold decreases in kcat value Methanocaldococcus jannaschii
Q61A the mutant shows an about 20fold decreases in kcat value Methanocaldococcus jannaschii
Y111F the mutation causes a 40% decrease in kcat value Methanocaldococcus jannaschii
Y78F the mutant shows an about 20fold decreases in kcat value Methanocaldococcus jannaschii

Inhibitors

Inhibitors Comment Organism Structure
additional information not inhibited by EDTA Methanocaldococcus jannaschii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0084
-
 7,8-dihydroneopterin mutant enzyme Q61A, at pH 7.6 and 70°C Methanocaldococcus jannaschii
0.0099
-
 7,8-dihydroneopterin wild type enzyme, at pH 7.6 and 70°C Methanocaldococcus jannaschii
0.011
-
 7,8-dihydroneopterin mutant enzyme Y111F, at pH 7.6 and 70°C Methanocaldococcus jannaschii
0.021
-
 7,8-dihydroneopterin mutant enzyme H35N, at pH 7.6 and 70°C Methanocaldococcus jannaschii
0.03
-
 7,8-dihydroneopterin mutant enzyme Y78F, at pH 7.6 and 70°C Methanocaldococcus jannaschii
0.0645
-
 7,8-dihydroneopterin wild type enzyme, at pH 7.6 and 25°C Methanocaldococcus jannaschii
0.25
-
 7,8-dihydroneopterin Km above 0.25 mM, mutant enzyme E25Q, at pH 7.6 and 70°C Methanocaldococcus jannaschii

Metals/Ions

Metals/Ions Comment Organism Structure
additional information the metal-independent enzyme is not influenced by Fe2+, Mn2+, Cu2+, Co2+, Ni2+, Mg2+, or Zn2+ Methanocaldococcus jannaschii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6-hydroxymethyl-7,8-dihydropterin + glycolaldehyde Methanocaldococcus jannaschii
-
 7,8-dihydroneopterin + 7,8-dihydromonapterin
-
 r
6-hydroxymethyl-7,8-dihydropterin + glycolaldehyde Methanocaldococcus jannaschii MJ0408
-
 7,8-dihydroneopterin + 7,8-dihydromonapterin
-
 r
7,8-dihydroneopterin Methanocaldococcus jannaschii
-
 6-(hydroxymethyl)-7,8-dihydropterin + glycolaldehyde
-
 r
7,8-dihydroneopterin Methanocaldococcus jannaschii MJ0408
-
 6-(hydroxymethyl)-7,8-dihydropterin + glycolaldehyde
-
 r

Organism

Organism UniProt Comment Textmining
Methanocaldococcus jannaschii
-
-
-
Methanocaldococcus jannaschii MJ0408
-
-
-

Purification (Commentary)

Purification (Comment) Organism
MonoQ anion exchange column chromatography and gel filtration Methanocaldococcus jannaschii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6-hydroxymethyl-7,8-dihydropterin + glycolaldehyde
-
 Methanocaldococcus jannaschii 7,8-dihydroneopterin + 7,8-dihydromonapterin
-
 r
6-hydroxymethyl-7,8-dihydropterin + glycolaldehyde
-
 Methanocaldococcus jannaschii MJ0408 7,8-dihydroneopterin + 7,8-dihydromonapterin
-
 r
7,8-dihydroneopterin
-
 Methanocaldococcus jannaschii 6-(hydroxymethyl)-7,8-dihydropterin + glycolaldehyde
-
 r
7,8-dihydroneopterin
-
 Methanocaldococcus jannaschii MJ0408 6-(hydroxymethyl)-7,8-dihydropterin + glycolaldehyde
-
 r

Subunits

Subunits Comment Organism
? x * 14000, SDS-PAGE Methanocaldococcus jannaschii

Synonyms

Synonyms Comment Organism
DHNA
-
 Methanocaldococcus jannaschii
MptD
-
 Methanocaldococcus jannaschii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
80
-
 the enzyme retains its activity following heating at 80°C for 20 min Methanocaldococcus jannaschii

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.048
-
 7,8-dihydroneopterin mutant enzyme Q61A, at pH 7.6 and 70°C Methanocaldococcus jannaschii
0.054
-
 7,8-dihydroneopterin mutant enzyme Y78F, at pH 7.6 and 70°C Methanocaldococcus jannaschii
0.055
-
 7,8-dihydroneopterin mutant enzyme H35N, at pH 7.6 and 70°C Methanocaldococcus jannaschii
0.07
-
 7,8-dihydroneopterin wild type enzyme, at pH 7.6 and 25°C Methanocaldococcus jannaschii
0.64
-
 7,8-dihydroneopterin mutant enzyme Y111F, at pH 7.6 and 70°C Methanocaldococcus jannaschii
0.68
-
 7,8-dihydroneopterin mutant enzyme E25Q, at pH 7.6 and 70°C Methanocaldococcus jannaschii
1
-
 7,8-dihydroneopterin wild type enzyme, at pH 7.6 and 70°C Methanocaldococcus jannaschii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 8.5
-
 Methanocaldococcus jannaschii

pH Stability

pH Stability pH Stability Maximum Comment Organism
10.5
-
 there is no protein aggregation or precipitation observed when proteins are incubated for 10 min at 70°C in 25mM tricine-CAPS-TES buffer at pH 10.5 Methanocaldococcus jannaschii

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1
-
 7,8-dihydroneopterin wild type enzyme, at pH 7.6 and 25°C Methanocaldococcus jannaschii
1.8
-
 7,8-dihydroneopterin mutant enzyme Y78F, at pH 7.6 and 70°C Methanocaldococcus jannaschii
2.6
-
 7,8-dihydroneopterin mutant enzyme H35N, at pH 7.6 and 70°C Methanocaldococcus jannaschii
2.7
-
 7,8-dihydroneopterin Km below 2.7 1/sec*mM, mutant enzyme E25Q, at pH 7.6 and 70°C Methanocaldococcus jannaschii
5.7
-
 7,8-dihydroneopterin mutant enzyme Q61A, at pH 7.6 and 70°C Methanocaldococcus jannaschii
58
-
 7,8-dihydroneopterin mutant enzyme Y111F, at pH 7.6 and 70°C Methanocaldococcus jannaschii
100
-
 7,8-dihydroneopterin wild type enzyme, at pH 7.6 and 70°C Methanocaldococcus jannaschii