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Literature summary for 4.1.2.32 extracted from

  • Parkin, K.L.; Hultin, H.O.
    Characterisation of trimethylamine-N-oxide (TMAO) demethylase activity from fish muscle microsomes (1986), J. Biochem., 100, 77-86.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
cysteine cofactor system composed of Fe2+, cysteine and/or ascorbate functions under aerobic and anaerobic conditions, a second cofactor system with NADH/FMN requires anaerobic conditions Urophycis chuss
additional information antagonistic relationship between two cofactor systems, implying competition for a single site Urophycis chuss

General Stability

General Stability Organism
inclusion of mercaptoethanol, EDTA and PMSF in the preparative buffers enhance yield and stability of microsomal activity Urophycis chuss

Inhibitors

Inhibitors Comment Organism Structure
ascorbate activity stimulated by NADH and FMN (anaerobic) is inhibited by 40% Urophycis chuss
cyanide K-salt of inhibits both cofactor systems by 75% Urophycis chuss
FeCl2 activity stimulated by NADH and FMN (anaerobic) is inhibited by 95% in the presence of 0.2 mM FeCl2, inhibition cannot be relieved by the presence of EDTA. Activity stimulated by ascorbate is inhibited by 94% in the presence of 0.2 mM FeCl2 Urophycis chuss
FMN 30% inhibition in the presence of Fe2+, cysteine, and ascorbate Urophycis chuss
iodoacetamide pre-incubation results in 25% loss of activity in both cofactor systems Urophycis chuss
additional information overview Urophycis chuss
NaN3 100% inhibition of NADH/FMN cofactor system (anaerobic conditions), 40% inhibition of the ascorbate/cysteine/Fe2+ cofactor system under anaerobic conditions Urophycis chuss
trimethylamine
-
Urophycis chuss

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7
-
Trimethylamine N-oxide catalyzed aerobically in presence of Fe2+ (0.2 mM), ascorbate (2 mM), and cysteine (2 mM) Urophycis chuss
21
-
Trimethylamine N-oxide catalyzed anaerobically in presence of FMN (0.1 mM) and NADH (0.4 mM) Urophycis chuss

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome
-
Urophycis chuss
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ cofactor system composed of Fe2+, cysteine and/or ascorbate functions under aerobic and anaerobic conditions, a second cofactor system with NADH/FMN requires anaerobic conditions Urophycis chuss
Fe2+ no activity when Fe2+ is replaced by the Cl-salts of Ca2+, Mn2+, Mg2+, Ni2+, Zn2+, or Co2+ for the ascorbate, cysteine, and Fe2+ cofactor system Urophycis chuss

Organism

Organism UniProt Comment Textmining
Urophycis chuss
-
red hake
-

Source Tissue

Source Tissue Comment Organism Textmining
muscle
-
Urophycis chuss
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Trimethylamine N-oxide
-
Urophycis chuss Dimethylamine + formaldehyde
-
?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
activity generated by both cofactor systems is lost Urophycis chuss
100
-
heating for 5 min deactivates partial purified fraction Urophycis chuss

Cofactor

Cofactor Comment Organism Structure
ascorbate cofactor system composed of Fe2+, cysteine and/or ascorbate functions under aerobic and anaerobic conditions, a second cofactor system with NADH/FMN requires anaerobic conditions Urophycis chuss
FMN cofactor system NADH/FMN requires anaerobic conditions, a second cofactor system composed of Fe2+, cysteine and/or ascorbate under aerobic or anaerobic conditions Urophycis chuss
NADH cofactor system NADH/FMN requires anaerobic conditions, a second cofactor system composed of Fe2+, cysteine and/or ascorbate under aerobic or anaerobic conditions Urophycis chuss