Activating Compound | Comment | Organism | Structure |
---|---|---|---|
cysteine | cofactor system composed of Fe2+, cysteine and/or ascorbate functions under aerobic and anaerobic conditions, a second cofactor system with NADH/FMN requires anaerobic conditions | Urophycis chuss | |
additional information | antagonistic relationship between two cofactor systems, implying competition for a single site | Urophycis chuss |
General Stability | Organism |
---|---|
inclusion of mercaptoethanol, EDTA and PMSF in the preparative buffers enhance yield and stability of microsomal activity | Urophycis chuss |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ascorbate | activity stimulated by NADH and FMN (anaerobic) is inhibited by 40% | Urophycis chuss | |
cyanide | K-salt of inhibits both cofactor systems by 75% | Urophycis chuss | |
FeCl2 | activity stimulated by NADH and FMN (anaerobic) is inhibited by 95% in the presence of 0.2 mM FeCl2, inhibition cannot be relieved by the presence of EDTA. Activity stimulated by ascorbate is inhibited by 94% in the presence of 0.2 mM FeCl2 | Urophycis chuss | |
FMN | 30% inhibition in the presence of Fe2+, cysteine, and ascorbate | Urophycis chuss | |
iodoacetamide | pre-incubation results in 25% loss of activity in both cofactor systems | Urophycis chuss | |
additional information | overview | Urophycis chuss | |
NaN3 | 100% inhibition of NADH/FMN cofactor system (anaerobic conditions), 40% inhibition of the ascorbate/cysteine/Fe2+ cofactor system under anaerobic conditions | Urophycis chuss | |
trimethylamine | - |
Urophycis chuss |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.7 | - |
Trimethylamine N-oxide | catalyzed aerobically in presence of Fe2+ (0.2 mM), ascorbate (2 mM), and cysteine (2 mM) | Urophycis chuss | |
21 | - |
Trimethylamine N-oxide | catalyzed anaerobically in presence of FMN (0.1 mM) and NADH (0.4 mM) | Urophycis chuss |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
microsome | - |
Urophycis chuss | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | cofactor system composed of Fe2+, cysteine and/or ascorbate functions under aerobic and anaerobic conditions, a second cofactor system with NADH/FMN requires anaerobic conditions | Urophycis chuss | |
Fe2+ | no activity when Fe2+ is replaced by the Cl-salts of Ca2+, Mn2+, Mg2+, Ni2+, Zn2+, or Co2+ for the ascorbate, cysteine, and Fe2+ cofactor system | Urophycis chuss |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Urophycis chuss | - |
red hake | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
muscle | - |
Urophycis chuss | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Trimethylamine N-oxide | - |
Urophycis chuss | Dimethylamine + formaldehyde | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
activity generated by both cofactor systems is lost | Urophycis chuss |
100 | - |
heating for 5 min deactivates partial purified fraction | Urophycis chuss |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ascorbate | cofactor system composed of Fe2+, cysteine and/or ascorbate functions under aerobic and anaerobic conditions, a second cofactor system with NADH/FMN requires anaerobic conditions | Urophycis chuss | |
FMN | cofactor system NADH/FMN requires anaerobic conditions, a second cofactor system composed of Fe2+, cysteine and/or ascorbate under aerobic or anaerobic conditions | Urophycis chuss | |
NADH | cofactor system NADH/FMN requires anaerobic conditions, a second cofactor system composed of Fe2+, cysteine and/or ascorbate under aerobic or anaerobic conditions | Urophycis chuss |