Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli strain BL21 Star (DE3) | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
D84G/DELTAY259 | the catalytic activity towards 2-deoxy-D-ribose-5-phosphate cleavage is increased 4fold compared to the wild type enzyme | Escherichia coli |
F200I | shows a nearly 14fold increase in productivity for (3R,5S)-6-chloro-2,4,6-trideoxyhexapyranoside formation | Escherichia coli |
K13C | shows a slight increase in productivity for (3R,5S)-6-chloro-2,4,6-trideoxyhexapyranoside formation | Escherichia coli |
M185T | shows a slight increase in productivity for (3R,5S)-6-chloro-2,4,6-trideoxyhexapyranoside formation | Escherichia coli |
M185V | mutation results in an about 5fold increase in (3R,5S)-6-chloro-2,4,6-trideoxyhexapyranoside formation compared to the wild type enzyme | Escherichia coli |
N80S/E127G/M185V/S258T/Y259T | contains an additional C-terminal KTQLSCTKW sequence, the catalytic activity towards 2-deoxy-D-ribose-5-phosphate cleavage is increased 2.5fold compared to the wild type enzyme | Escherichia coli |
S239C | shows a slight increase in productivity for (3R,5S)-6-chloro-2,4,6-trideoxyhexapyranoside formation | Escherichia coli |
S93G/A174V | shows a nearly 3fold increase in productivity for (3R,5S)-6-chloro-2,4,6-trideoxyhexapyranoside formation | Escherichia coli |
T19I/I166T | shows a slight increase in productivity for (3R,5S)-6-chloro-2,4,6-trideoxyhexapyranoside formation | Escherichia coli |
T19S | shows a slight increase in productivity for (3R,5S)-6-chloro-2,4,6-trideoxyhexapyranoside formation | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Chloroacetaldehyde | the enzyme is rapidly and irreversibly inactivated and loses 84.9% of its enzymatic activity in the presence of 200 mM chloroacetaldehyde | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
24 | - |
Chloroacetaldehyde | mutant enzyme F200I | Escherichia coli | |
54.6 | - |
Chloroacetaldehyde | wild type enzyme | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-4-chloro-3-hydroxybutanal + acetaldehyde | - |
Escherichia coli | (3R,5S)-6-chloro-2,4,6-trideoxyhexapyranoside | - |
? | |
2-deoxy-D-ribose 5-phosphate | - |
Escherichia coli | D-glyceraldehyde 3-phosphate + acetaldehyde | - |
r | |
chloroacetaldehyde + acetaldehyde | - |
Escherichia coli | (S)-4-chloro-3-hydroxybutanal | - |
? |
Synonyms | Comment | Organism |
---|---|---|
2-deoxy-D-ribose 5-phosphate aldolase | - |
Escherichia coli |
DERA | - |
Escherichia coli |