Application | Comment | Organism |
---|---|---|
synthesis | stereoselectivity is induced into aldol reactions of this naturally promiscuous enzyme by employing D-glyceraldehyde acetonide and L-glyceraldehyde acetonide as substrates | Saccharolobus solfataricus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.9 | - |
D-glyceraldehyde | pH 6, 70°C | Saccharolobus solfataricus | |
5.5 | - |
D-glyceraldehyde acetonide | pH 6, 70°C | Saccharolobus solfataricus | |
7.1 | - |
L-glyceraldehyde | pH 6, 70°C | Saccharolobus solfataricus | |
22.7 | - |
D-glyceraldehyde acetonide | pH 6, 70°C | Saccharolobus solfataricus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharolobus solfataricus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
pyruvate + D-glyceraldehyde | the enzyme exhibits no diastereocontrol for the aldol condensation of its natural substrates pyruvate and D-glyceraldehyde and gives a 50:50 mixture of 2-dehydro-3-deoxy-D-gluconate (anti-(4S,5R)-3-deoxy-2-hexulosonate) and 2-dehydro-3-deoxy-D-galactobate (syn-(4R,5R)-3-deoxy-2-hexulosonate) | Saccharolobus solfataricus | 2-dehydro-3-deoxy-D-galactonate | - |
? | |
pyruvate + D-glyceraldehyde | the enzyme exhibits no diastereocontrol for the aldol condensation of its natural substrates pyruvate and D-glyceraldehyde and gives a 50:50 mixture of 2-dehydro-3-deoxy-D-gluconate (anti-(4S,5R)-3-deoxy-2-hexulosonate) and 2-dehydro-3-deoxy-D-galactobate (syn-(4R,5R)-3-deoxy-2-hexulosonate) | Saccharolobus solfataricus | 2-dehydro-3-deoxy-D-gluconate | - |
? | |
pyruvate + D-glyceraldehyde acetonide | highly stereoselective formation of 3-deoxy-5,6-O-(1-methylethylidene)-D-erythro-hex-2-ulosonate in 92% diastereomeric excess | Saccharolobus solfataricus | 3-deoxy-5,6-O-(1-methylethylidene)-D-erythro-hex-2-ulosonate | - |
? | |
pyruvate + L-glyceraldehyde | condensation of pyruvate with L-glyceraldehyde proceeds with no stereocontrol, yielding a 53:47 diastereoisomeric mixture of 2-dehydro-3-deoxy-L-gluconate (anti-(4S,5R)-3-deoxy-2-hexulosonate) and 2-dehydro-3-deoxy-L-galactonate (syn-(4S,5S)-3-deoxy-2-hexulosonate) | Saccharolobus solfataricus | 2-dehydro-3-deoxy-L-galactonate | - |
? | |
pyruvate + L-glyceraldehyde | condensation of pyruvate with L-glyceraldehyde proceeds with no stereocontrol, yielding a 53:47 diastereoisomeric mixture of 2-dehydro-3-deoxy-L-gluconate (anti-(4S,5R)-3-deoxy-2-hexulosonate) and 2-dehydro-3-deoxy-L-galactonate (syn-(4S,5S)-3-deoxy-2-hexulosonate) | Saccharolobus solfataricus | 2-dehydro-3-deoxy-L-gluconate | - |
? | |
pyruvate + L-glyceraldehyde acetonide | highly stereoselective formation of 3-deoxy-5,6-O-(1-methylethylidene)-L-threo-hex-2-ulosonate in 94% diastereomeric excess | Saccharolobus solfataricus | 3-deoxy-5,6-O-(1-methylethylidene)-L-threo-hex-2-ulosonate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
2-keto-3-deoxygluconate aldolase | - |
Saccharolobus solfataricus |
KDGA | - |
Saccharolobus solfataricus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.7 | - |
D-glyceraldehyde acetonide | pH 6, 70°C | Saccharolobus solfataricus | |
5.4 | - |
D-glyceraldehyde acetonide | pH 6, 70°C | Saccharolobus solfataricus | |
9.9 | - |
D-glyceraldehyde | pH 6, 70°C | Saccharolobus solfataricus | |
9.9 | - |
L-glyceraldehyde | pH 6, 70°C | Saccharolobus solfataricus |