Any feedback?
Literature summary for 4.1.3.1 extracted from
- Isolation and characterization of isocitrate lyase and malate synthase from Bacillus stearothermophilus (1975), Biochem. Soc. Trans., 3, 303-306.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| KCl | strong activation by KCl | Geobacillus stearothermophilus |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.021 | - |
threo-Ds-isocitrate | - |
Geobacillus stearothermophilus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 48000 | - |
4 * 48000, SDS-PAGE | Geobacillus stearothermophilus |
| 180000 | - |
gel filtration | Geobacillus stearothermophilus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| isocitrate | Geobacillus stearothermophilus | glyoxylate cycle | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Geobacillus stearothermophilus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Geobacillus stearothermophilus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 2.9 | - |
- |
Geobacillus stearothermophilus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (2R,3S)-isocitrate | - |
Geobacillus stearothermophilus | succinate + glyoxylate | - |
? | |
| isocitrate | - |
Geobacillus stearothermophilus | succinate + glyoxylate | - |
r | |
| isocitrate | glyoxylate cycle | Geobacillus stearothermophilus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 48000, SDS-PAGE | Geobacillus stearothermophilus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
50% activity lost after 4 h | Geobacillus stearothermophilus |
| 60 | - |
50% activity lost after 3 min | Geobacillus stearothermophilus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 24 | - |
isocitrate | - |
Geobacillus stearothermophilus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Geobacillus stearothermophilus |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
