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Literature summary for 4.1.3.1 extracted from
- The purification and physicochemical characterization of maize (Zea mays L.) isocitrate lyase (1992), Arch. Biochem. Biophys., 297, 9-18.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ba2+ | no inhibition | Zea mays |  |
| Ca2+ | no inhibition | Zea mays | |
| Sr2+ | no inhibition | Zea mays | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.06 | - |
isocitrate | 100 mM Hepes, pH 7.0 | Zea mays | |
| 0.52 | - |
isocitrate | 60 mM potassium phosphate, pH 7.5 | Zea mays | |
| 0.76 | - |
isocitrate | 60 mM triethanolamine, pH 7.5 | Zea mays | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ba2+ | no activation | Zea mays | |
| Mg2+ | activity of demetallized enzyme: 0.5% | Zea mays | |
| Mn2+ | Mn2+ 30% as effective as Mg2+ | Zea mays | |
| Sr2+ | no activation | Zea mays | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 64000 | - |
4 * 64000, SDS-PAGE | Zea mays |
| 252000 | - |
gel filtration | Zea mays |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| isocitrate | Zea mays | - |
? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Zea mays | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| no modification | - |
Zea mays |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Zea mays |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| scutellum | - |
Zea mays | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 4.96 | - |
- |
Zea mays |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -70°C, concentrated to 2.4 mg/ml, 10 mM triethanolamine buffer, 12 mM MgCl2, 14 mM 2-mercaptoethanol, pH 7.6, 50% v/v glycerol, several months, stable | Zea mays |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| isocitrate | - |
Zea mays | succinate + glyoxylate | - |
r | |
| isocitrate | - |
Zea mays | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 64000, SDS-PAGE | Zea mays |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.2 | - |
- |
Zea mays |
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