Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 4.1.3.1 extracted from

  • Tanaka, Y.; Yoshida, T.; Watanabe, K.; Izumi, Y.; Mitsunaga, T.
    Characterization, gene cloning and expression of isocitrate lyase involved in the assimilation of one-carbon compounds in Hyphomicrobium methylovorum GM2 (1997), Eur. J. Biochem., 249, 820-825.
    View publication on PubMed
Search for more literature for 4.1.3.1

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Hyphomicrobium methylovorum

Inhibitors

Inhibitors Comment Organism Structure
Ag+
-
 Hyphomicrobium methylovorum
Al3+
-
 Hyphomicrobium methylovorum
Cd2+
-
 Hyphomicrobium methylovorum
DL-penicillamin
-
 Hyphomicrobium methylovorum
glycolate 20% inhibition at 1 mM Hyphomicrobium methylovorum
iodoacetamide
-
 Hyphomicrobium methylovorum
KCl
-
 Hyphomicrobium methylovorum
KCN
-
 Hyphomicrobium methylovorum
Li+
-
 Hyphomicrobium methylovorum
Mn2+
-
 Hyphomicrobium methylovorum
NaCl
-
 Hyphomicrobium methylovorum
oxalate 77% inhibition at 1 mM Hyphomicrobium methylovorum
Sr2+
-
 Hyphomicrobium methylovorum
succinate 38% inhibition at 1 mM Hyphomicrobium methylovorum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.051
-
 threo-Ds-isocitrate
-
 Hyphomicrobium methylovorum

Metals/Ions

Metals/Ions Comment Organism Structure
Ba2+ no activation Hyphomicrobium methylovorum
Mg2+ increase of activity Hyphomicrobium methylovorum
Mn2+ Mn2+ 27% as effective as Mg2+ Hyphomicrobium methylovorum
Mn2+ no activity in absence of exogenous cation Hyphomicrobium methylovorum
Sr2+ no activation Hyphomicrobium methylovorum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
62000
-
 4 * 62000, SDS-PAGE Hyphomicrobium methylovorum
250000
-
 gel filtration Hyphomicrobium methylovorum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
isocitrate Hyphomicrobium methylovorum assimilation of one-carbon compounds in the icl+ serine pathway ?
-
 ?
isocitrate Hyphomicrobium methylovorum glyoxylate cycle ?
-
 ?

Organism

Organism UniProt Comment Textmining
Hyphomicrobium methylovorum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Hyphomicrobium methylovorum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4
-
-
 Hyphomicrobium methylovorum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2R,3S)-isocitrate
-
 Hyphomicrobium methylovorum succinate + glyoxylate
-
 ?
isocitrate
-
 Hyphomicrobium methylovorum succinate + glyoxylate
-
 ?
isocitrate assimilation of one-carbon compounds in the icl+ serine pathway Hyphomicrobium methylovorum ?
-
 ?
isocitrate glyoxylate cycle Hyphomicrobium methylovorum ?
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 62000, SDS-PAGE Hyphomicrobium methylovorum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
 Hyphomicrobium methylovorum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
25
-
 no loss of activity after 30 min Hyphomicrobium methylovorum
30
-
 16% loss of activity after 30 min Hyphomicrobium methylovorum
40
-
 30% loss of activity after 30 min Hyphomicrobium methylovorum
50
-
 82% loss of activity after 30 min Hyphomicrobium methylovorum
60
-
 100% loss of activity after 30 min Hyphomicrobium methylovorum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Hyphomicrobium methylovorum

pH Stability

pH Stability pH Stability Maximum Comment Organism
7.5 9 at 15°C stable for 30 min Hyphomicrobium methylovorum