KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.51 | - |
DL-isocitrate | 20°C, pH 6.8 | Colwellia maris |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | can partly replace Mg2+ | Colwellia maris | |
Mg2+ | 10 mM, dependent | Colwellia maris | |
Mn2+ | can partly replace Mg2+ | Colwellia maris | |
Ni2+ | can partly replace Mg2+ | Colwellia maris |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
64700 | - |
4 * 64700, SDS-PAGE | Colwellia maris |
240000 | - |
gel filtration | Colwellia maris |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
isocitrate | Colwellia maris | - |
succinate + glyoxylate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Colwellia maris | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Colwellia maris |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
6.17 | - |
- |
Colwellia maris |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
isocitrate | - |
Colwellia maris | succinate + glyoxylate | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 64700, SDS-PAGE | Colwellia maris |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | - |
- |
Colwellia maris |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
10 | - |
half maximal enzyme activity | Colwellia maris |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
enzyme is rapidly inactivated above, NaCl, 10% glycerol and 1 mM dithiothreitol protects | Colwellia maris |
35 | - |
no enzyme activity | Colwellia maris |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
18.1 | - |
DL-isocitrate | 20°C, pH 6.8 | Colwellia maris |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.8 | - |
- |
Colwellia maris |