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Literature summary for 4.1.3.1 extracted from
- Amino acid residues involved in cold adaptation of isocitrate lyase from a psychrophilic bacterium, Colwellia maris (2004), Microbiology, 150, 3393-3403.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Colwellia maris |
| expression in Escherichia coli | Escherichia coli |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| Q207H | mutant with decreased activity between 10°C and 25°C | Colwellia maris |
| Q217K | mutant with decreased activity between 10°C and 25°C | Colwellia maris |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.25 | - |
isocitrate | 10°C | Escherichia coli |  |
| 0.26 | - |
isocitrate | mutant Q217K, 10°C | Colwellia maris | |
| 0.33 | - |
isocitrate | wild-type, 10°C | Colwellia maris | |
| 0.47 | - |
isocitrate | wild-type, 20°C | Colwellia maris | |
| 0.5 | - |
isocitrate | mutant Q217K, 30°C | Colwellia maris | |
| 0.65 | - |
isocitrate | 40°C | Escherichia coli | |
| 0.76 | - |
isocitrate | mutant Q207H, 10°C | Colwellia maris | |
| 2.07 | - |
isocitrate | mutant Q207H, 30°C | Colwellia maris | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Colwellia maris | - |
- |
- |
| Escherichia coli | P0A9G6 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Colwellia maris |
- |
Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 10.5 | - |
mutant Q217K | Colwellia maris |
| 14.5 | - |
mutant Q207H | Colwellia maris |
| 14.6 | - |
wild-type | Colwellia maris |
| 54 | - |
- |
Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| isocitrate | - |
Colwellia maris | glyoxylate + succinate | - |
? | |
| isocitrate | - |
Escherichia coli | glyoxylate + succinate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ICL | - |
Colwellia maris |
| ICL | - |
Escherichia coli |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
optimum temperature above 40°C | Escherichia coli |
| 20 | 25 | wild type | Colwellia maris |
| 27.5 | 30 | mutant Q207H | Colwellia maris |
| 30 | 32.5 | mutant Q217K | Colwellia maris |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
mutant Q207H, half time of thermal inactivation 58.4 s | Colwellia maris |
| 30 | - |
mutant Q217K, half time of thermal inactivation 120.3 s | Colwellia maris |
| 30 | - |
wild-type, half time of thermal inactivation 88.2 s | Colwellia maris |
| 40 | - |
half time of thermal inactivation 161.8 s | Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.8 | - |
wild-type | Colwellia maris |
| 6.85 | - |
mutants Q207H and Q217k | Colwellia maris |
| 6.9 | - |
- |
Escherichia coli |
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