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Literature summary for 4.1.3.1 extracted from

  • Gould, T.A.; van de Langemheen, H.; Munoz-Elias, E.J.; McKinney, J.D.; Sacchettini, J.C.
    Dual role of isocitrate lyase 1 in the glyoxylate and methylcitrate cycles in Mycobacterium tuberculosis (2006), Mol. Microbiol., 61, 940-947.
    View publication on PubMed
Search for more literature for 4.1.3.1

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Mycobacterium tuberculosis

Crystallization (Commentary)

Crystallization (Comment) Organism
vapour diffusion with 0.1 M Tris-HCl, 0.2 M sodium acetate, 20-30% (w/v) PEG 4000, pH 8.0 Mycobacterium tuberculosis

Protein Variants

Protein Variants Comment Organism
C191A unable to form the closed conformation crystal form Mycobacterium tuberculosis
C195A reduced activity Mycobacterium tuberculosis
C195S reduced activity Mycobacterium tuberculosis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.188
-
 isocitrate ICL1, in 50 mM MOPS pH 6.8, 5 mM MgCl2 Mycobacterium tuberculosis
0.718
-
 (2R,3S:2S,3R)-2-methyl-isocitrate ICL1, in 50 mM MOPS pH 6.8, 5 mM MgCl2 Mycobacterium tuberculosis
1.14
-
 isocitrate ICL2, in Tricine-HCl pH 7.5, 5 mM MgCl2 Mycobacterium tuberculosis

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Mycobacterium tuberculosis

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis P9WKK7
-
-
Mycobacterium tuberculosis H37Rv P9WKK7
-
-

Purification (Commentary)

Purification (Comment) Organism
HiTrap Q FF ion exchange column chromatography, Superdex S-200 gel filtration, and HiTrap Q HP ion exchange column chromatography Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2R,3S:2S,3R)-2-methyl-isocitrate
-
 Mycobacterium tuberculosis succinate + pyruvate
-
 ?
(2R,3S:2S,3R)-2-methyl-isocitrate
-
 Mycobacterium tuberculosis H37Rv succinate + pyruvate
-
 ?
isocitrate
-
 Mycobacterium tuberculosis succinate + glyoxylate
-
 ?
isocitrate
-
 Mycobacterium tuberculosis H37Rv succinate + glyoxylate
-
 ?

Subunits

Subunits Comment Organism
homotetramer x-ray crystallography Mycobacterium tuberculosis

Synonyms

Synonyms Comment Organism
ICL1 isoenzyme, has both isocitrate lyase and 2-methylisocitrate lyase activity Mycobacterium tuberculosis
ICL2 isoenzyme, has no 2-methylisocitrate lyase activity Mycobacterium tuberculosis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.25
-
 (2R,3S:2S,3R)-2-methyl-isocitrate ICL1, in 50 mM MOPS pH 6.8, 5 mM MgCl2 Mycobacterium tuberculosis
1.38
-
 isocitrate ICL2, in Tricine-HCl pH 7.5, 5 mM MgCl2 Mycobacterium tuberculosis
5.24
-
 isocitrate ICL1, in 50 mM MOPS pH 6.8, 5 mM MgCl2 Mycobacterium tuberculosis