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Literature summary for 4.1.3.1 extracted from

  • Eprintsev, A.T.; Fedorin, D.N.; Salnikov, A.V.; Igamberdiev, A.U.
    Expression and properties of the glyoxysomal and cytosolic forms of isocitrate lyase in Amaranthus caudatus L (2015), J. Plant Physiol., 181, 1-8 .
    View publication on PubMed
Search for more literature for 4.1.3.1

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ isoforms ICL1 and ICL2 are 60-80% inhibited by 5 mM Cu2+ Amaranthus caudatus
Fe2+ isoforms ICL1 and ICL2 are 30% inhibited by 5 mM Fe2+ Amaranthus caudatus
Mg2+ isoform ICL2 is 20% inhibited by 5 mM Mg2+ Amaranthus caudatus
Mn2+ isoform ICL1 is 40% inhibited by 5 mM Mn2+ Amaranthus caudatus
Zn2+ isoforms ICL1 and ICL2 are 60-80% inhibited by 5 mM Zn2+ Amaranthus caudatus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.045
-
 DL-isocitrate isoform ICL1, with 5 mM MgCl2, at pH 7.5 and 25°C Amaranthus caudatus
0.063
-
 DL-isocitrate isoform ICL2, with 5 mM MgCl2, at pH 7.5 and 25°C Amaranthus caudatus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol isoform ICL2 Amaranthus caudatus 5829
-
glyoxysome isoform ICL1 Amaranthus caudatus 9514
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ isoform ICL1 is activated 3fold by 5 mM Mg2+ Amaranthus caudatus
Mn2+ isoform ICL2 is activated 3fold by 5 mM Mn2+ Amaranthus caudatus
additional information isoform ICL2 is not activated by Mg2+. Ca2+and K+ reveal no marked effect on both isoforms of the enzyme Amaranthus caudatus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
isocitrate Amaranthus caudatus
-
 succinate + glyoxylate
-
 r

Organism

Organism UniProt Comment Textmining
Amaranthus caudatus
-
 cultivar Kinelskiy 254
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, DEAE cellulose column chromatography, and Sephadex G-25 gel filtration Amaranthus caudatus

Source Tissue

Source Tissue Comment Organism Textmining
seedling
-
 Amaranthus caudatus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.024
-
 isoform ICL2, at pH 6.5 and 25°C Amaranthus caudatus
0.03
-
 isoform ICL1, with 5 mM MnCl2, at pH 6.5 and 25°C Amaranthus caudatus
0.035
-
 isoform ICL2, with 5 mM MnCl2, at pH 6.5 and 25°C Amaranthus caudatus
0.48
-
 isoform ICL1, at pH 6.5 and 25°C Amaranthus caudatus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DL-isocitrate
-
 Amaranthus caudatus succinate + glyoxylate
-
 r
isocitrate
-
 Amaranthus caudatus succinate + glyoxylate
-
 r

Synonyms

Synonyms Comment Organism
D-threo-isocitrate: glyoxylate lyase
-
 Amaranthus caudatus
ICL1 isoform Amaranthus caudatus
ICL2 isoform Amaranthus caudatus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
 isoform ICL2 Amaranthus caudatus
7.5
-
 isoform ICL1 Amaranthus caudatus

Expression

Organism Comment Expression
Amaranthus caudatus the highest isoform ICL1 activity is observed on the 3rd day of germination; then the activity and expression of the corresponding gene decreases, while the activity of isoform ICL2 and gene expression increases to the 7th day of germination and then remains at the same level up

General Information

General Information Comment Organism
metabolism the function of isoform ICL1 is related to the glyoxylate cycle while isoform ICL2 functions independently from the glyoxylate cycle and interconverts organic acids in the cytosol Amaranthus caudatus