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Literature summary for 4.1.3.16 extracted from
- The metabolism of gamma-hydroxyglutamate in rat liver. 1. Enzymic synthesis of gamma-hydroxy-alpha-ketoglutarate from pyruvate and glyoxylate (1963), Biochim. Biophys. Acta, 78, 617-628.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| arsenite | - |
Rattus norvegicus |  |
| Cu2+ | 93% inhibition at 2 mM | Rattus norvegicus | |
| cysteine | 65% inhibition at 10 mM | Rattus norvegicus | |
| Hg2+ | complete inhibition at 2 mM | Rattus norvegicus | |
| iodoacetate | 10% inhibition at 10 mM | Rattus norvegicus | |
| N-ethylmaleimide | 64% inhibition at 10 mM | Rattus norvegicus | |
| p-chloromercuribenzoate | complete inhibition at 10 mM | Rattus norvegicus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.43 | - |
glyoxylate | - |
Rattus norvegicus | |
| 1.33 | - |
4-hydroxy-alpha-ketoglutarate | - |
Rattus norvegicus | |
| 10 | - |
pyruvate | - |
Rattus norvegicus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| mitochondrion | - |
Rattus norvegicus | 5739 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no requirement | Rattus norvegicus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
rat | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | poor activity | Rattus norvegicus | - |
| kidney | - |
Rattus norvegicus | - |
| liver | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 5.3 | - |
- |
Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-Hydroxy-2-oxoglutarate | - |
Rattus norvegicus | Pyruvate + glyoxylate | - |
? | |
| Glyoxylate + pyruvate | specific, codensation favoured, Keq: 0.73 mM | Rattus norvegicus | 4-Hydroxy-2-ketoglutarate | - |
? | |
| additional information | specific for glyoxylate and pyruvate | Rattus norvegicus | ? | - |
? | |
| additional information | equilibrium favours condensation, Keq: 0.73 1/mM | Rattus norvegicus | ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
enzyme assay at | Rattus norvegicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | 8 | condensation, elimination | Rattus norvegicus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 9 | - |
Rattus norvegicus |
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Release 2023.1 (January 2023)
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