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Literature summary for 4.1.3.4 extracted from
- Partial purification and characterization of membrane-associated 3-hydroxy-3-methylglutaryl coenzyme A lyase from radish seedlings (1993), Z. Naturforsch. C, 48, 444-450.
No PubMed abstract available
General Stability
| General Stability | Organism |
|---|---|
| extremely unstable | Raphanus sativus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.04 | - |
hydroxymethylglutaryl-CoA | - |
Raphanus sativus |  |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | - |
Raphanus sativus | 16020 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Bivalent cations | - |
Raphanus sativus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 70000 | - |
gel filtration | Raphanus sativus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (S)-3-Hydroxy-3-methylglutaryl-CoA | Raphanus sativus | involved in regulation of isoprenoid pathway | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Raphanus sativus | - |
radish | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Raphanus sativus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| seedling | - |
Raphanus sativus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.0098 | - |
- |
Raphanus sativus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -80°C, several weeks | Raphanus sativus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (S)-3-Hydroxy-3-methylglutaryl-CoA | involved in regulation of isoprenoid pathway | Raphanus sativus | ? | - |
? | |
| 3-hydroxy-3-methylglutaryl-CoA | - |
Raphanus sativus | acetoacetate + acetyl-CoA | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
rapidly inactivated above | Raphanus sativus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Raphanus sativus |
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