Activating Compound | Comment | Organism | Structure |
---|---|---|---|
dithiothreitol | activates 2fold at 5 mM | Pseudomonas mevalonii |
Cloned (Comment) | Organism |
---|---|
- |
Pseudomonas mevalonii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(R)-3-hydroxy-3-methylglutaryl-CoA | - |
Pseudomonas mevalonii | |
1,10-phenanthroline | 43% activity after 4 h at 4 mM | Pseudomonas mevalonii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
(S)-3-hydroxy-3-methylglutaryl-CoA | pH 8.2, 30°C, recombinant enzyme | Pseudomonas mevalonii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | tightly bound to the enzyme | Pseudomonas mevalonii | |
Mg2+ | increases activity at millimolar concentrations | Pseudomonas mevalonii | |
Mn2+ | increases activity at micromolar concentrations | Pseudomonas mevalonii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
64000 | - |
calculated from Stokes' radius | Pseudomonas mevalonii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Pseudomonas mevalonii | last step in ketogenesis | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas mevalonii | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas mevalonii |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
70 | - |
- |
Pseudomonas mevalonii |
Storage Stability | Organism |
---|---|
-80°C, retains full activity for several months in phosphate buffer containing 20% glycerol at pH 7.2 | Pseudomonas mevalonii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-3-Hydroxy-3-methylglutaryl-CoA | - |
Pseudomonas mevalonii | Acetyl-CoA + acetoacetate | - |
ir | |
additional information | last step in ketogenesis | Pseudomonas mevalonii | ? | - |
? |