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Literature summary for 4.1.3.43 extracted from

  • Wang, W.; Baker, P.; Seah, S.Y.
    Comparison of two metal-dependent pyruvate aldolases related by convergent evolution: substrate specificity, kinetic mechanism, and substrate channeling (2010), Biochemistry, 49, 3774-3782.
    View publication on PubMed
Search for more literature for 4.1.3.43

Inhibitors

Inhibitors Comment Organism Structure
2-oxobutanoate competitive inhibition Paraburkholderia xenovorans
2-oxopentanoate competitive inhibition Paraburkholderia xenovorans
4-methyl-2-oxopentanoate competitive inhibition Paraburkholderia xenovorans
glyoxylate competitive inhibition Paraburkholderia xenovorans
pyruvate competitive inhibition Paraburkholderia xenovorans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
14.8
-
 pyruvate steady-state kinetic parameter, pH 8.0 and 25°C Paraburkholderia xenovorans
64.3
-
 acetaldehyde app. Km-value with pyruvate as carbonyl donor, pH 8.0 and 25°C Paraburkholderia xenovorans
67
-
 acetaldehyde steady-state kinetic parameter, pH 8.0 and 25°C Paraburkholderia xenovorans
124.6
-
 glycolaldehyde app. Km-value with pyruvate as carbonyl donor, pH 8.0 and 25°C Paraburkholderia xenovorans
135.9
-
 propionaldehyde app. Km-value with pyruvate as carbonyl donor, pH 8.0 and 25°C Paraburkholderia xenovorans

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ cofactor Paraburkholderia xenovorans

Organism

Organism UniProt Comment Textmining
Paraburkholderia xenovorans
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
pyruvate + acetaldehyde
-
 Paraburkholderia xenovorans (4S)-4-hydroxy-2-oxopentanoate
-
 ?
pyruvate + butyraldehyde
-
 Paraburkholderia xenovorans ?
-
 ?
pyruvate + glycolaldehyde
-
 Paraburkholderia xenovorans ?
-
 ?
pyruvate + isobutyraldehyde
-
 Paraburkholderia xenovorans ?
-
 ?
pyruvate + pentaldehyde
-
 Paraburkholderia xenovorans ?
-
 ?
pyruvate + propionaldehyde
-
 Paraburkholderia xenovorans ?
-
 ?

Synonyms

Synonyms Comment Organism
BphI
-
 Paraburkholderia xenovorans

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.4
-
 glycolaldehyde app. kcat-value with pyruvate as carbonyl donor, pH 8.0 and 25°C Paraburkholderia xenovorans
0.86
-
 acetaldehyde app. kcat-value with pyruvate as carbonyl donor, pH 8.0 and 25°C Paraburkholderia xenovorans
1.04
-
 pyruvate steady-state kinetic parameter, with acetaldehyde as aldehyde donor, pH 8.0 and 25°C Paraburkholderia xenovorans
1.79
-
 propionaldehyde app. kcat-value with pyruvate as carbonyl donor, pH 8.0 and 25°C Paraburkholderia xenovorans

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.21
-
 glyoxylate pH 8.0, 25°C Paraburkholderia xenovorans
0.34
-
 2-oxobutanoate pH 8.0, 25°C Paraburkholderia xenovorans
0.55
-
 pyruvate pH 8.0, 25°C Paraburkholderia xenovorans
1.09
-
 2-oxopentanoate pH 8.0, 25°C Paraburkholderia xenovorans
2.71
-
 4-methyl-2-oxopentanoate pH 8.0, 25°C Paraburkholderia xenovorans
10.3
-
 pyruvate pH 8.0, 25°C Paraburkholderia xenovorans

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.00047
-
 pentaldehyde kcat(app)/Km(app) with pyruvate as carbonyl donor, pH 8.0 and 25°C Paraburkholderia xenovorans
0.0005
-
 Isobutyraldehyde kcat(app)/Km(app) with pyruvate as carbonyl donor, pH 8.0 and 25°C Paraburkholderia xenovorans
0.0022
-
 Butyraldehyde kcat(app)/Km(app) with pyruvate as carbonyl donor, pH 8.0 and 25°C Paraburkholderia xenovorans
0.0037
-
 glycolaldehyde kcat(app)/Km(app) with pyruvate as carbonyl donor, pH 8.0 and 25°C Paraburkholderia xenovorans
0.0132
-
 propionaldehyde kcat(app)/Km(app) with pyruvate as carbonyl donor, pH 8.0 and 25°C Paraburkholderia xenovorans
0.0134
-
 acetaldehyde kcat(app)/Km(app) with pyruvate as carbonyl donor, pH 8.0 and 25°C Paraburkholderia xenovorans