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Literature summary for 4.1.3.6 extracted from

  • Bekal, S.; van Beeumen, J.; Samyn, B.; Garmyn, D.; Henini, S.; Divies, C.; Prevost, H.
    Purification of Leuconostoc mesenteroides citrate lyase and cloning and characterization of the citCDEFG gene cluster (1998), J. Bacteriol., 180, 647-654.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.1.3.6

Cloned(Commentary)

Cloned (Comment) Organism
expression of the citCDEFG gene cluster in Escherichia coli. The gene product of citD is the gamma-subunit of citrate lyase. The gene product of citE is the beta-subunit of citrate lyase. The gene product of citF is the alpha-subunit of citrate lyase. The enzyme synthesized in Escherichia coli lacks its prosthetic group and is active only in the presence of acetyl-CoA Leuconostoc mesenteroides

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
14000
-
 x * 14000 + x * 34000 + x * 55000, SDS-PAGE Leuconostoc mesenteroides
34000
-
 x * 14000 + x * 34000 + x * 55000, SDS-PAGE Leuconostoc mesenteroides
55000
-
 x * 14000 + x * 34000 + x * 55000, SDS-PAGE Leuconostoc mesenteroides

Organism

Organism UniProt Comment Textmining
Leuconostoc mesenteroides
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Leuconostoc mesenteroides

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Leuconostoc mesenteroides

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
citrate
-
 Leuconostoc mesenteroides acetate + oxaloacetate
-
 ?

Subunits

Subunits Comment Organism
oligomer x * 14000 + x * 34000 + x * 55000, SDS-PAGE Leuconostoc mesenteroides

Synonyms

Synonyms Comment Organism
More can be dissociated into components, two of which are identical with ec 2.8.3.10 and ec 4.1.3. 34 Leuconostoc mesenteroides