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Literature summary for 4.1.3.6 extracted from
- Characterization of citrate lyase from Clostridium sporosphaeroides (1985), Arch. Microbiol., 141, 85-90.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| L-Glu | addition of L-Glu is not necessary for activity and stabilization of the enzyme | [Clostridium] sporosphaeroides |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.24 | - |
citrate | - |
[Clostridium] sporosphaeroides | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | divalent cation required: Mg2+, Zn2+, Mn2+, Co2+ or Cu2+ | [Clostridium] sporosphaeroides | |
| Cu2+ | divalent cation required: Mg2+, Zn2+, Mn2+, Co2+ or Cu2+ | [Clostridium] sporosphaeroides | |
| Mg2+ | divalent cation required: Mg2+, Zn2+, Mn2+, Co2+ or Cu2+, Mg2+ is most effective | [Clostridium] sporosphaeroides | |
| Mn2+ | divalent cation required: Mg2+, Zn2+, Mn2+, Co2+ or Cu2+ | [Clostridium] sporosphaeroides | |
| Zn2+ | divalent cation required: Mg2+, Zn2+, Mn2+, Co2+ or Cu2+ | [Clostridium] sporosphaeroides | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 11800 | - |
x * 11800, + x * 34000 + x * 56600, SDS-PAGE | [Clostridium] sporosphaeroides |
| 34000 | - |
x * 11800, + x * 34000 + x * 56600, SDS-PAGE | [Clostridium] sporosphaeroides |
| 56600 | - |
x * 11800, + x * 34000 + x * 56600, SDS-PAGE | [Clostridium] sporosphaeroides |
| 536600 | - |
gel filtration, sedimentation equilibrium centrifugation | [Clostridium] sporosphaeroides |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| [Clostridium] sporosphaeroides | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
[Clostridium] sporosphaeroides |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
[Clostridium] sporosphaeroides |
| 34.1 | - |
- |
[Clostridium] sporosphaeroides |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, pH 7.2, 100 mM potassium phosphate buffer, 3 mM MgCl2, 1 mM dithiothreitol, stable for 3 days | [Clostridium] sporosphaeroides |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| citrate | - |
[Clostridium] sporosphaeroides | acetate + oxaloacetate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| oligomer | x * 11800, + x * 34000 + x * 56600, SDS-PAGE | [Clostridium] sporosphaeroides |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
[Clostridium] sporosphaeroides |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
24 h, 7% loss of activity | [Clostridium] sporosphaeroides |
| 60 | - |
15 min, 9% loss of activity | [Clostridium] sporosphaeroides |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.8 | 8 | in 100 mM tris-HCl buffer or 100 mM potassium phosphate buffer | [Clostridium] sporosphaeroides |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.8 | 9 | pH 6.8-8.0: pH-optimum, pH 9.0: more than 80% of maximal activity | [Clostridium] sporosphaeroides |
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