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Literature summary for 4.1.99.1 extracted from

  • Kogan, A.; Gdalevsky, G.Y.; Cohen-Luria, R.; Goldgur, Y.; Phillips, R.S.; Parola, A.H.; Almog, O.
    Conformational changes and loose packing promote E. coli tryptophanase cold lability (2009), BMC Struct. Biol., 9, 65.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.1.99.1

Crystallization (Commentary)

Crystallization (Comment) Organism
mutant enzymes Y74F and C298S, hanging drop vapor diffusion method, using 30% (w/v) PEG 400, 100 mM HEPES pH 7.5, 200 mM MgCl2, 5 mM 2-mercaptoethanol Escherichia coli

Protein Variants

Protein Variants Comment Organism
C298S the mutant displays reduced activity, subsequent to incubation at 2°C, the mutant Trpase loses about 90% of its activity Escherichia coli
W330F the mutant displays reduced activity, subsequent to incubation at 2°C, the mutant Trpase loses about 90% of its activity Escherichia coli
Y74F the mutant displays reduced activity, the Y74F mutant has low activity at 25°C and its residual activity is further reduced by cooling Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli P0A853
-
-
Escherichia coli SVS 370 P0A853
-
-

Purification (Commentary)

Purification (Comment) Organism
DEAE-Sephadex A-50 gel filtration or DEAE-Sepharose 4B column chromatography, and Sephacryl S-300 gel filtration Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-Trp + H2O
-
 Escherichia coli indole + pyruvate + NH4+
-
 ?
L-Trp + H2O
-
 Escherichia coli SVS 370 indole + pyruvate + NH4+
-
 ?

Subunits

Subunits Comment Organism
tetramer x-ray crystallography Escherichia coli

Synonyms

Synonyms Comment Organism
Trpase
-
 Escherichia coli
tryptophan indole-lyase
-
 Escherichia coli

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2
-
 subsequent to incubation at 2°C, wild type Trpase loses about 35% of its activity followed by dissociation into dimers. Complete dissociation/inactivation occurs after incubation for 20 h at 2°C and association/reactivation occurs upon warming for few hours Escherichia coli

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
 Escherichia coli