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Literature summary for 4.1.99.1 extracted from

  • Yoshida, Y.; Sasaki, T.; Ito, S.; Tamura, H.; Kunimatsu, K.; Kato, H.
    Identification and molecular characterization of tryptophanase encoded by tnaA in Porphyromonas gingivalis (2009), Microbiology, 155, 968-978.
    View publication on PubMed
Search for more literature for 4.1.99.1

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Porphyromonas gingivalis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.09
-
 L-Trp purified recombinant tryptophanase in 200 mM sodium phosphate buffer (pH 7.5), at 37°C Porphyromonas gingivalis
0.2
-
 L-Trp purified recombinant tryptophanase in 200 mM potassium phosphate buffer (pH 7.5), at 37°C Porphyromonas gingivalis
0.26
-
 S-ethyl-L-cysteine purified recombinant tryptophanase in 200 mM potassium phosphate buffer (pH 7.5), at 37°C Porphyromonas gingivalis
2.04
-
 S-methyl-L-cysteine purified recombinant tryptophanase in 200 mM potassium phosphate buffer (pH 7.5), at 37°C Porphyromonas gingivalis

Metals/Ions

Metals/Ions Comment Organism Structure
additional information TnaA is not affected by K+ or Na+ Porphyromonas gingivalis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
52500
-
 calculated from amino acid sequence Porphyromonas gingivalis

Organism

Organism UniProt Comment Textmining
Porphyromonas gingivalis
-
-
-
Porphyromonas gingivalis W83
-
-
-

Purification (Commentary)

Purification (Comment) Organism
glutathione-Sepharose column chromatography Porphyromonas gingivalis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-Trp + H2O
-
 Porphyromonas gingivalis indole + pyruvate + NH4+
-
 ?
L-Trp + H2O
-
 Porphyromonas gingivalis W83 indole + pyruvate + NH4+
-
 ?
additional information tryptophanase does not degrade L-alanine, L-serine or L-cysteine Porphyromonas gingivalis ?
-
 ?
additional information tryptophanase does not degrade L-alanine, L-serine or L-cysteine Porphyromonas gingivalis W83 ?
-
 ?
S-ethyl-L-cysteine + H2O
-
 Porphyromonas gingivalis ?
-
 ?
S-ethyl-L-cysteine + H2O
-
 Porphyromonas gingivalis W83 ?
-
 ?
S-methyl-L-cysteine + H2O
-
 Porphyromonas gingivalis ?
-
 ?
S-methyl-L-cysteine + H2O
-
 Porphyromonas gingivalis W83 ?
-
 ?

Synonyms

Synonyms Comment Organism
TnaA
-
 Porphyromonas gingivalis
tryptophan indole-lyase
-
 Porphyromonas gingivalis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40 70 the enzyme is stable up to 40°C, but only 40% of the activity remains at 60°C, the enzyme is completely inactivated at temperatures above 70°C Porphyromonas gingivalis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.24
-
 L-Trp purified recombinant tryptophanase in 200 mM sodium phosphate buffer (pH 7.5), at 37°C Porphyromonas gingivalis
0.5
-
 S-methyl-L-cysteine purified recombinant tryptophanase in 200 mM potassium phosphate buffer (pH 7.5), at 37°C Porphyromonas gingivalis
1.37
-
 L-Trp purified recombinant tryptophanase in 200 mM potassium phosphate buffer (pH 7.5), at 37°C Porphyromonas gingivalis
1.87
-
 S-ethyl-L-cysteine purified recombinant tryptophanase in 200 mM potassium phosphate buffer (pH 7.5), at 37°C Porphyromonas gingivalis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Porphyromonas gingivalis

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
 Porphyromonas gingivalis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.25
-
 S-methyl-L-cysteine purified recombinant tryptophanase in 200 mM potassium phosphate buffer (pH 7.5), at 37°C Porphyromonas gingivalis
6.87
-
 L-Trp purified recombinant tryptophanase in 200 mM potassium phosphate buffer (pH 7.5), at 37°C Porphyromonas gingivalis
7.15
-
 S-ethyl-L-cysteine purified recombinant tryptophanase in 200 mM potassium phosphate buffer (pH 7.5), at 37°C Porphyromonas gingivalis