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Literature summary for 4.1.99.1 extracted from

  • Phillips, R.S.; Kalu, U.; Hay, S.
    Evidence of preorganization in quinonoid intermediate formation from L-Trp in H463F mutant Escherichia coli tryptophan indole-lyase from effects of pressure and pH (2012), Biochemistry, 51, 6527-6533.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
H463F site-directed mutagenesis, the mutant shows very low activity for elimination of indole but is still competent to form a quinonoid intermediate from L-tryptophan, pH dependence of quinonoid intermediate formation, overview Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information kinetics of mutant H463F, high-pressure stopped-flow measurements, overview Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-tryptophan + H2O Escherichia coli
-
indole + pyruvate + NH3
-
r

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Reaction

Reaction Comment Organism Reaction ID
L-tryptophan + H2O = indole + pyruvate + NH3 quinonoid intermediate formation involving Tyr71 and Cys298, mechanism, active site preorganization, overview Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-tryptophan + H2O
-
Escherichia coli indole + pyruvate + NH3
-
r
L-tryptophan + H2O reversible hydrolytic beta-elimination Escherichia coli indole + pyruvate + NH3
-
r
S-(2-nitrophenyl)-L-cysteine + H2O
-
Escherichia coli ?
-
r

Synonyms

Synonyms Comment Organism
TIL
-
Escherichia coli
tryptophan indole-lyase
-
Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
22
-
assay at room temperature Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Escherichia coli

pH Range

pH Minimum pH Maximum Comment Organism
6.5 9.5 pH dependence of quinonoid intermediate formation, overview Escherichia coli

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
Escherichia coli