Protein Variants | Comment | Organism |
---|---|---|
H463F | site-directed mutagenesis, the mutant shows very low activity for elimination of indole but is still competent to form a quinonoid intermediate from L-tryptophan, it shows high activity with substrate beta-(benzimidazol-1-yl)-L-alanine | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-amino-4-(benzimidazol-1-yl)butyric acid | i.e. homo-BZI-Ala, a potent competitive inhibitor | Escherichia coli | |
2-amino-5-(benzimidazol-1-yl)pentanoic acid | i.e. bishomo-BZI-Ala, weak inhibition | Escherichia coli | |
beta-(benzimidazol-1-yl)-L-alanine | competitive, is also a good substrate for the wild-type and mutant enzymes | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | pre-steady-state and steady-state kinetics of wild-type and mutant enzymes, overview | Escherichia coli | |
0.0236 | - |
beta-(benzimidazol-1-yl)-L-alanine | mutant H463F, pH 8.0, 25°C | Escherichia coli | |
0.15 | - |
L-tryptophan | wild-type enzyme, pH 8.0, 25°C | Escherichia coli | |
0.315 | - |
beta-(benzimidazol-1-yl)-L-alanine | wild-type enzyme, pH 8.0, 25°C | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
52000 | - |
x * 52000, wild-type enzyme, SDS-PAGE | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-tryptophan + H2O | Escherichia coli | - |
indole + pyruvate + NH3 | - |
? | |
L-tryptophan + H2O | Escherichia coli JM109 | - |
indole + pyruvate + NH3 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
gene tnaA on plasmid pMD6 | - |
Escherichia coli JM109 | - |
gene tnaA on plasmid pMD6 | - |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
L-tryptophan + H2O = indole + pyruvate + NH3 | reaction mechanism, overview | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-(benzimidazol-1-yl)-L-alanine + H2O | reaction via aldimine intermediate, high activity with wild-type enzyme and mutant H463F | Escherichia coli | ? | - |
? | |
beta-(benzimidazol-1-yl)-L-alanine + H2O | reaction via aldimine intermediate, high activity with wild-type enzyme and mutant H463F | Escherichia coli JM109 | ? | - |
? | |
L-tryptophan + H2O | - |
Escherichia coli | indole + pyruvate + NH3 | - |
? | |
L-tryptophan + H2O | - |
Escherichia coli JM109 | indole + pyruvate + NH3 | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 52000, wild-type enzyme, SDS-PAGE | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
TIL | - |
Escherichia coli |
tryptophan indole lyase | - |
Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.22 | - |
beta-(benzimidazol-1-yl)-L-alanine | mutant H463F, pH 8.0, 25°C | Escherichia coli | |
4 | - |
L-tryptophan | wild-type enzyme, pH 8.0, 25°C | Escherichia coli | |
5.6 | - |
beta-(benzimidazol-1-yl)-L-alanine | wild-type enzyme, pH 8.0, 25°C | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Escherichia coli |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0134 | - |
2-amino-4-(benzimidazol-1-yl)butyric acid | wild-type enzyme, pH 8.0, 25°C | Escherichia coli | |
0.3 | - |
beta-(benzimidazol-1-yl)-L-alanine | wild-type enzyme, pH 8.0, 25°C | Escherichia coli | |
0.6 | - |
2-amino-5-(benzimidazol-1-yl)pentanoic acid | above, wild-type enzyme, pH 8.0, 25°C | Escherichia coli |
General Information | Comment | Organism |
---|---|---|
physiological function | tryptophan indole lyase produces indole from L-tryptophan, indole is a signaling molecule in bacteria, affecting biofilm formation, pathogenicity and antibiotic resistance | Escherichia coli |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
9.3 | - |
beta-(benzimidazol-1-yl)-L-alanine | mutant H463F, pH 8.0, 25°C | Escherichia coli | |
18.67 | - |
beta-(benzimidazol-1-yl)-L-alanine | wild-type enzyme, pH 8.0, 25°C | Escherichia coli | |
27 | - |
L-tryptophan | wild-type enzyme, pH 8.0, 25°C | Escherichia coli |